Short food deprivation inhibits orexin receptor 1 expression and orexin-A induced intracellular calcium signaling in acutely isolated duodenal enterocytes

American Journal of Physiology. Gastrointestinal and Liver Physiology
M W BengtssonG Flemström

Abstract

Close intra-arterial infusion of the appetite regulating peptide orexin-A stimulates bicarbonate secretion from the duodenal mucosa. The aim of the present study was to elucidate the ability of orexin-A to induce intracellular calcium signaling in acutely isolated duodenal enterocytes. Freshly isolated clusters of enterocytes, obtained from rat duodenal mucosa or human duodenal biopsies, were loaded with fura 2-AM and mounted in a perfusion chamber. Cryptlike enterocytes were selected (caged), and changes in intracellular calcium concentration ([Ca2+]i) were evaluated by fluorescence imaging. Total RNA was extracted from pellets of enterocytes and reverse transcribed to cDNA, and expression of orexin receptors 1 and 2 (OX1R and OX2R) was measured by quantitative real-time PCR. Orexin-A at all concentrations tested (1-100 nM) increased [Ca2+]i in enterocytes isolated from continuously fed rats, and the OX1R-antagonist SB-334867 (10 nM) attenuated the response. The primary [Ca2+]i response was a slow increase to a sustained plateau persisting after orexin-A removal, and a similar response was observed in enterocytes from human biopsies. In contrast to orexin-A, the OX2R agonist (Ala11,D-Leu15)-orexin-B (1-10 nM) did not induce ca...Continue Reading

References

Feb 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·L de LeceaJ G Sutcliffe
Aug 4, 1998·The American Journal of Physiology·C S ChewG Flemström
Jan 12, 1999·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·J R Pappenheimer
Jan 28, 1999·Biochemical and Biophysical Research Communications·N TakahashiY Kohgo
Jan 7, 2000·Neuron·A L Kirchgessner, M Liu
Jul 6, 2000·The Journal of Biological Chemistry·P E LundK E Akerman
Nov 22, 2000·Journal of Biochemical and Biophysical Methods·T D Schmittgen, B A Zakrajsek
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·M J BerridgeM D Bootman
Aug 21, 2001·Biochemical and Biophysical Research Communications·B BeckA Stricker-Krongrad
Dec 19, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·María J Sánchez de Miguel, María A Burrell
Feb 14, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·E NäslundA L Kirchgessner
Sep 5, 2002·FEBS Letters·Tomas HolmqvistJyrki P Kukkonen
Sep 27, 2002·European Journal of Pharmacology·Katsuhisa MatsuoYoshibumi Nakane
Nov 1, 2002·Pancreas·Kyoko MiyasakaAkihiro Funakoshi
Nov 7, 2002·American Journal of Physiology. Cell Physiology·Jyrki P KukkonenKarl E O Akerman
Dec 7, 2002·Bioorganic & Medicinal Chemistry Letters·Shuichi AsahiHajime Morishima
Feb 14, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Markus SjöblomGunnar Flemström
Jul 3, 2003·Nature Reviews. Molecular Cell Biology·Michael J BerridgeH Llewelyn Roderick
Jul 4, 2003·American Journal of Physiology. Cell Physiology·Stephen A KempsonMarshall H Montrose
Aug 2, 2003·Molecular and Cellular Endocrinology·Masao NakabayashiHironobu Sasano
Aug 29, 2003·Biochemical and Biophysical Research Communications·K P LarssonK H Herzig
Nov 13, 2004·The Journal of Biological Chemistry·Kim P LarssonKarl E O Akerman
Dec 14, 2004·American Journal of Physiology. Cell Physiology·Adrian Allen, Gunnar Flemström
Feb 3, 2005·American Journal of Physiology. Endocrinology and Metabolism·Emmanouil KarterisHarpal S Randeva
Mar 3, 2005·Histochemistry and Cell Biology·Hans-Rudolf BerthoudHuiyuan Zheng
Oct 25, 2006·Scandinavian Journal of Gastroenterology·Bengt SäfstenGunnar Flemström
Jun 23, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Magnus W BengtssonGunnar Flemström
Feb 26, 2008·Acta Physiologica·M V HeinonenK H Herzig

❮ Previous
Next ❯

Citations

Jun 6, 2009·Acta Physiologica·H TsunekiT Sasaoka
Sep 2, 2009·Acta Physiologica·M LaburtheA El Firar
Nov 10, 2012·Journal of Pharmacological and Toxicological Methods·Katsutoshi IdoKohei Sawada
Jul 28, 2015·Molecular and Cellular Endocrinology·Johan FernøMiguel López
Jun 24, 2017·Current Protocols in Pharmacology·Beatrice KisserMarkus Keiser

❮ Previous
Next ❯

Related Concepts

Related Feeds

Asprosin

Asprosin is a fasting-induced hormone produced in the white adipose tissue to stimulate the hepatic release of glucose into the bloodstream. Discover the latest research on this protein hormone here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.