Short interfering RNA accumulation correlates with host recovery in DNA virus-infected hosts, and gene silencing targets specific viral sequences

Journal of Virology
Padmanabhan ChellappanClaude M Fauquet

Abstract

Viruses are both inducers and targets of posttranscriptional gene silencing (PTGS), a natural defense mechanism in plants. Here we report molecular evidence of the ability of single-stranded DNA (ssDNA) viruses to induce PTGS in infected plants irrespective of the severity of or recovery from the symptoms. Our results reveal that five distinct species of cassava-infecting geminiviruses were capable of triggering PTGS by producing two classes of virus-specific short interfering RNAs (siRNAs) of 21 to 26 nucleotides in two plant hosts, tobacco (Nicotiana benthamiana) and cassava (Manihot esculenta, Crantz). However, the efficacy of virus-induced PTGS varied depending on the intrinsic features of the virus and its interaction with the plant host. We found that symptom recovery over time in plants infected with the isolates of African cassava mosaic virus (ACMV-[CM]) or Sri Lankan cassava mosaic virus was associated with a much higher level of virus-derived siRNA accumulation compared to plants infected with viruses that do not show symptom recovery. Furthermore, we determined that the C terminus of AC1 that overlaps with the N terminus of AC2 early viral genes involved in virus replication were the primary targets for ACMV-[CM]-in...Continue Reading

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Citations

Jan 5, 2005·Plant Molecular Biology·Padmanabhan ChellappanClaude M Fauquet
Oct 23, 2012·Molecular Biotechnology·Namisha SharmaManoj Prasad
Oct 17, 2013·Nature Reviews. Microbiology·Nathan Pumplin, Olivier Voinnet
Nov 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·Alfred W BronkhorstRonald P van Rij
Jan 20, 2006·Nucleic Acids Research·Rashid AkbergenovMikhail M Pooggin
Nov 9, 2006·Nucleic Acids Research·Todd BlevinsMikhail M Pooggin
Jun 23, 2011·Molecular Plant-microbe Interactions : MPMI·Rajiv Kumar Yadav, Debasis Chattopadhyay
Jun 17, 2006·Molecular Plant-microbe Interactions : MPMI·Xue-Yu BianM Ali Rezaian
Jun 9, 2007·Molecular Plant-microbe Interactions : MPMI·Kenny BonfimFrancisco J L Aragão
Oct 24, 2008·Phytopathology·Jimena Carrillo-TrippRafael F Rivera-Bustamante
Jul 26, 2005·Plant Physiology·Padmanabhan ChellappanClaude M Fauquet
Jun 16, 2005·Journal of Virology·P V ShivaprasadMikhail M Pooggin
Aug 7, 2013·Annual Review of Phytopathology·Xueping Zhou
Aug 5, 2005·Annual Review of Phytopathology·Robin MacDiarmid
Jun 21, 2006·Annual Review of Phytopathology·D FargetteJ M Thresh
Aug 6, 2008·Annual Review of Phytopathology·Juan A Díaz-Pendón, Shou-Wei Ding
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Nov 29, 2012·Virology Journal·Elsa Góngora-CastilloRafael F Rivera-Bustamante
Oct 3, 2012·PLoS Pathogens·Michael AreggerMikhail M Pooggin
Jul 28, 2013·International Journal of Molecular Sciences·Mikhail M Pooggin
Jul 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Padmanabhan ChellappanClaude M Fauquet
Dec 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Guillaume Moissiard, Olivier Voinnet
Jun 13, 2009·Annual Review of Plant Biology·Virginia Ruiz-Ferrer, Olivier Voinnet
Mar 8, 2005·Trends in Plant Science·Ramachandran VanitharaniClaude M Fauquet
Jun 6, 2009·FEBS Letters·Muhammad Shah Nawaz-ul-Rehman, Claude M Fauquet
Jun 21, 2011·Biochimica Et Biophysica Acta·Thomas Hohn, Franck Vazquez
May 10, 2011·Biochimica Et Biophysica Acta·Hanako Shimura, Vitantonio Pantaleo

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