Short-interfering RNA-mediated silencing of thioredoxin reductase 1 alters the sensitivity of HeLa cells toward cadmium

Biological & Pharmaceutical Bulletin
Michie NishimotoShuntaro Hara

Abstract

The mammalian thioredoxin reductase (TrxR) is a selenocysteine-containing flavoprotein that regulates the thioredoxin system, one of the major systems that maintain the intracellular redox balance. We previously reported that cytosolic TrxR (TrxR1), one of three mammalian TrxR isozymes, was induced by treatment with cadmium. In the present study, to study the role of cadmium-induced TrxR1 in cellular defense, we silenced the expression of TrxR1 in HeLa cells by using small interfering RNA and examined the effect of TrxR1 silencing on the sensitivity of the cells toward cadmium. We found that the gene silencing of TrxR1 had a dual effect on cadmium-induced cell death, depending on the concentration of cadmium. The TrxR1 silencing increased the sensitivity toward a low dose (less than 10 microM) of cadmium but decreased the sensitivity toward a high dose of cadmium. These results suggested that TrxR1 might play an important role in the cellular defense system against cadmium in two ways. TrxR1 might rescue the cells from a low dose of cadmium-induced moderate injury, while it might promote the death of cells severely injured by a high dose of cadmium.

References

Apr 1, 1992·Biological Trace Element Research·G N Schrauzer
Jan 1, 1997·Annual Review of Immunology·H NakamuraJ Yodoi
Aug 24, 1999·The Journal of Biological Chemistry·Q A SunV N Gladyshev
Nov 24, 1999·Free Radical Biology & Medicine·R Brigelius-Flohé
Feb 3, 2000·Free Radical Biology & Medicine·F Liu, K Y Jan
Dec 1, 2001·Free Radical Biology & Medicine·J Nordberg, E S Arnér
Nov 21, 2002·The Journal of Biological Chemistry·Daniel StewartJawed Alam
Jan 11, 2003·Free Radical Biology & Medicine·Hirotaka Imai, Yasuhito Nakagawa
Oct 3, 2003·The Journal of Biological Chemistry·Hiroaki MurataTakahito Kondo
Oct 30, 2003·Journal of Cellular Physiology·Atsuko SakuraiShuntaro Hara

❮ Previous
Next ❯

Citations

Sep 22, 2009·Free Radical Biology & Medicine·Sofi E ErikssonElias S J Arnér
Jul 27, 2007·Basic & Clinical Pharmacology & Toxicology·Greicy M M ConteratoTatiana Emanuelli
Dec 19, 2013·Basic & Clinical Pharmacology & Toxicology·Greicy M M ConteratoTatiana Emanuelli
Jan 30, 2013·Critical Reviews in Biochemistry and Molecular Biology·Young-Mi Go, Dean P Jones
Aug 30, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Geir BjørklundMassimiliano Peana

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.