Short interfering RNA targeting Net1 reduces the angiogenesis and tumor growth of in vivo cervical squamous cell carcinoma through VEGF down-regulation

Human Pathology
Yuting ZhangEnlin Song

Abstract

Net1, a guanine nucleotide exchange factor, is implicated in cancer cell invasion through activation of RhoA. However, there is still no report on the association between Net1 and cancer angiogenesis. The current study was designed to explore the role of Net1 in the angiogenesis of cervical squamous cell carcinoma (CSCC) and further observe the effects of Net1 short interfering RNA (siRNA) on the tumor growth. Net1 was overexpressed in CSCC samples (n=80), correlated with the cancer microvessel density (r=0.223, P=.026), and related to aggressive clinical behaviors, including depth of cervical wall invasion (P=.041), parametrial involvement (P=.037), lymph node metastasis (P=.021), and vascular invasion (P=.018). Human umbilical vein endothelial cells treated with supernatant of SiHa cells with Net1 siRNA showed significantly decreased proliferation (0.75±0.038 versus 1.0±0.015, P<.001), migration (39.3±6.5 versus 66.0±10.1, P=.019), and tube formation (13.5±3.05 versus 21.7±2.89, P=.030) compared with those human umbilical vein endothelial cells treated with normal SiHa cells supernatant. Net1 siRNA of SiHa decreased VEGF expression level (0.60±0.026 versus 0.78±0.031, P=.02). Furthermore, Net1 siRNA significantly reduced tumo...Continue Reading

References

Mar 17, 2000·Biochimica Et Biophysica Acta·V SerruE Rubinstein
Sep 23, 2000·Nature·P Carmeliet, R K Jain
Feb 13, 2002·The Journal of Biological Chemistry·Anja Schmidt, Alan Hall
Mar 9, 2002·Advances in Cancer Research·Aron B Jaffe, Alan Hall
Jul 13, 2002·International Journal of Cancer. Journal International Du Cancer·Volker WollscheidMatthias Dürst
Dec 13, 2002·Nature·Sandrine Etienne-Manneville, Alan Hall
Mar 15, 2003·Nature Reviews. Cancer·Erik Sahai, Christopher J Marshall
May 16, 2003·Current Biology : CB·Marc Symons, Nicole Rusk
Mar 11, 2004·Differentiation; Research in Biological Diversity·Akira MiyakoshiNoriyuki Kinoshita
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Kent L RossmanJohn Sondek
Apr 10, 2010·Gynecologic Oncology·Song En-linWang Hua-qiao
Oct 11, 2011·The Journal of Pathology·Tanguy Lechertier, Kairbaan Hodivala-Dilke
May 31, 2012·Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine·Xue HanLi-Li Tang
May 3, 2013·Journal of Molecular Histology·Yuan-Yuan WuYuan-Yuan Zhu
Jun 12, 2013·International Journal of Oncology·Sebastian HöltersIgnacio Rubio
Aug 16, 2013·Journal of Experimental & Clinical Cancer Research : CR·Conor LahiffDavid Murray
Nov 8, 2013·Archives of Dermatological Research·Jie zhangXingyu Li
Apr 30, 2016·Cellular Oncology (Dordrecht)·Roman Paduch
Aug 9, 2016·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Joan J CastellanoMariano Monzo
Jan 7, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Julien LesageMyriam Polette

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Citations

Aug 28, 2019·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Wei ZongRongrong Jing
May 28, 2020·Cancers·Ángela ÁyenHouria Boulaiz
Apr 16, 2021·Advanced Drug Delivery Reviews·Xue WangZhigang Xie

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