Short Link N promotes disc repair in a rabbit model of disc degeneration

Arthritis Research & Therapy
F MwaleJ Antoniou

Abstract

The degeneration of the intervertebral disc (IVD) is characterized by proteolytic degradation of the extracellular matrix, and its repair requires the production of an extracellular matrix with a high proteoglycan-to-collagen ratio characteristic of a nucleus pulposus (NP)-like phenotype in vivo. At the moment, there is no medical treatment to reverse or even retard disc degeneration. The purpose of the present study was to determine if a low dose of short link N (sLN), a recently discovered fragment of the link N peptide, could behave in a manner similar to that of link N in restoring the proteoglycan content and proteoglycan-to-collagen ratio of the disc in a rabbit model of IVD degeneration, as an indication of its potential therapeutic benefit in reversing disc degeneration. Adolescent New Zealand white rabbits received an annular puncture with an 18-gauge needle into two noncontiguous discs to induce disc degeneration. Two weeks later, either saline (10 μL) or sLN (25 μg in 10 μL saline) was injected into the center of the NP. The sLN concentration was empirically chosen at a lower molar concentration equivalent to half that of link N (100 μg in 10 μL). The effect on radiographic, biochemical and histologic changes were ev...Continue Reading

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Citations

Nov 12, 2020·Experimental Biology and Medicine·Demissew Shenegelegn MernClaudius Thomé
Apr 25, 2021·Acta Biomaterialia·João F Crispim, Keita Ito

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Methods Mentioned

BETA
dissection
X-ray

Software Mentioned

MATLAB
GraphPad
GraphPad Prism

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