Short N-terminal deletions in the phage phi 29 transcriptional activator protein impair its DNA-binding ability

Gene
Fernando Rojo, M Salas

Abstract

The expression of Bacillus subtilis phage phi 29 late genes from the A3 promoter requires the viral protein p4. This protein is a transcriptional activator which binds to a region of the A3 promoter located between nucleotides -56 to -102, relative to the transcription start point. Mutants at the N terminus of protein p4 have been constructed and their function investigated. The binding of these deletion mutants to the late A3 promoter has been analyzed by gel retardation and DNase I footprinting assays. The results indicate that the N terminus of protein p4 could be involved in its binding to the A3 promoter, suggesting that it may not be a typical Cro-like helix-turn-helix DNA-binding protein.

References

Jan 5, 1990·Journal of Molecular Biology·J U Bowie, R T Sauer
Jul 20, 1989·Journal of Molecular Biology·I Barthelemy, M Salas
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K L Knight, R T Sauer
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E SanteroS Kustu
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·Y L RenJ S Krakow
Sep 2, 1988·Science·R Schleif
Jan 1, 1986·Gene·Y MizukamiH Hirokawa
Dec 1, 1986·Journal of Virology·I BarthelemyR P Mellado
Sep 20, 1986·Journal of Molecular Biology·R P MelladoM Salas
Jan 1, 1988·Annual Review of Biophysics and Biophysical Chemistry·D P Goldenberg
Jan 1, 1984·Annual Review of Biochemistry·C O Pabo, R T Sauer
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert

❮ Previous
Next ❯

Citations

Feb 1, 1996·American Journal of Physical Anthropology·C ManzanoC de la Rúa
Apr 1, 1994·American Journal of Physical Anthropology·P MoralG Vona
Sep 1, 1991·Research in Microbiology·F RojoM Salas
May 7, 2013·Surgical Neurology International·Ha Son NguyenLaurie L Ackerman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.