PMID: 8598297Mar 15, 1996Paper

Short-range transcriptional repressors mediate both quenching and direct repression within complex loci in Drosophila

Genes & Development
S Gray, Michael Levine

Abstract

The early Drosophila embryo provides a unique system for the analysis of transcriptional repression since a broad spectrum of repressors are distributed in spatially distinct patterns. Krüppel (Kr) and snail (sna), two zinc finger repressors, are essential for segmentation and for the establishment of the mesoderm/neuroectoderm boundary, respectively. Both repressors were examined in the context of synthetic gene complexes containing modular promoters and divergently transcribed reporter genes. These studies indicate that Kr and sna function as short-range repressors, which can mediate either quenching or direct repression of the transcription complex, depending on the location of repressor sites. When located within an upstream enhancer, the repressor locally quenches nearby activators and permits other enhancers to interact with the transcription complex (enhancer autonomy). In contrast, when bound to promoter-proximal regions the repressor functions in a dominant fashion and blocks multiple enhancers. Local quenching and dominant repression require close linkage (<100 bp) of the repressor with either upstream activators or the transcription complex. These studies establish short-range repression as a flexible form of gene re...Continue Reading

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Citations

Jan 12, 1999·Developmental Genetics·Z P Xu, G F Saunders
Oct 12, 2010·Cellular and Molecular Life Sciences : CMLS·Johannes Jaeger
Nov 16, 2002·Current Opinion in Genetics & Development·Michael Z Ludwig
Jul 3, 2013·Nature Methods·Justin Crocker, David L Stern
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Aug 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Michael W PerryMichael Levine
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·H N CaiM Levine
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Mar 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Albert Erives, Michael Levine
Feb 3, 2016·Nature Communications·Michal Brunwasser-MeiromRoee Amit
Oct 22, 2008·Current Opinion in Genetics & Development·Stefan Bonn, Eileen E M Furlong

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