Apr 13, 2020

Jchain-driven cre enables specific genetic manipulation and timestamping of plasma cell in their niche.

BioRxiv : the Preprint Server for Biology
A. Q. XuDinis Pedro Calado

Abstract

Plasma cells produce antibodies that are essential for protection from infection and for vaccination success. Plasma cells are also at the origin of currently incurable hematological malignancies, most notably multiple myeloma. However, the study of gene function in plasma cells has mostly been performed using in vitro cultures and cell transfer systems that remove plasma cells from their microenvironment. Further, existing systems to genetically manipulate plasma cells are not specific and target multiple cell lineages including B cells, making it difficult to discriminate gene function in plasma cell formation and/or maintenance. Here we characterized a genetically engineered allele: IgjcreERT2(GFP) that expresses GFP and creERT2 under the activity of the endogenous Jchain promoter. We found at the single cell level that Jchain expression occurred in plasma cells across immunoglobulin isotypes. Also using the IgjcreERT2(GFP) we performed highly specific cre-loxP genetic manipulation of plasma cells residing in their natural microenvironment in vivo in the mouse and traced long-lived plasma cells.

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Mentioned in this Paper

Study
Patterns
RCHY1 gene
Genome
Gene Deletion
Pan troglodytes
DNA Resequencing
Nucleotides
Structure
Species

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