Short-term adrenalectomy increases glucocorticoid and mineralocorticoid receptor mRNA in selective areas of the developing hippocampus

Molecular and Cellular Neurosciences
D M VázquezH Akil

Abstract

In the central nervous system, adrenal steroids bind to two different types of corticosteroid receptors: glucocorticoid (GR) or mineralocorticoid (MR). In vitro biochemical and autoradiographic techniques have been used to infer GR and MR protein abundance in the hippocampus. Adrenalectomy (ADX) is routinely performed to measure the normal receptor number in absence of corticosterone (B), which would otherwise interfere with the binding reaction, The developing rodent has low basal B levels until the third week of life. We were interested in whether removal of circulating B may have a greater impact in the developing hippocampus than in the adult animal. In this study we examined the effect of a 14-h ADX on hippocampal GR and MR binding capacity (B(max)) by standard binding techniques and on gene expression by in situ hybridization. ADX was performed on Day 6, 10, 14, 18, 22, 28, 35, and 45 and on adult animals. GR B(max) increased from Day 6 to adult levels by Day 22 (d6 = 159.0 +/- 27; d22 = 369.1 +/- 43; a = 344.8 +/- 23, fmol/mg protein +/- SE). In contrast, MR B(max) had adult levels on Day 6 and increased above these until Day 45, when it decreased and approached adult concentrations (d6 = 83.2 +/- 22; d45 = 123 +/- 23; a...Continue Reading

Citations

Oct 27, 1998·Stress : the International Journal on the Biology of Stress·D L HelmreichS J Watson
May 1, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Charles R NealDelia M Vázquez
Dec 18, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Shelly B FlagelCharles R Neal
Oct 26, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·T BrabhamD M Vázquez
Dec 17, 1998·Psychoneuroendocrinology·D M Vázquez

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