Short-Term Depression of Axonal Spikes at the Mouse Hippocampal Mossy Fibers and Sodium Channel-Dependent Modulation

ENeuro
Shunsuke Ohura, Haruyuki Kamiya

Abstract

Axonal spike is an important upstream process of transmitter release, which directly impacts on release probability from the presynaptic terminals. Despite the functional significance, possible activity-dependent modulation of axonal spikes has not been studied extensively, partly due to inaccessibility of the small structures of axons for electrophysiological recordings. In this study, we tested the possibility of use-dependent changes in axonal spikes at the hippocampal mossy fibers, where direct recordings from the axon terminals are readily feasible. Hippocampal slices were made from mice of either sex, and loose-patch clamp recordings were obtained from the visually identified giant mossy fiber boutons located in the stratum lucidum of the CA3 region. Stimulation of the granule cell layer of the dentate gyrus elicited axonal spikes at the single bouton which occurred in all or none fashion. Unexpected from the digital nature of spike signaling, the peak amplitude of the second spikes in response to paired stimuli at a 50-ms interval was slightly but reproducibly smaller than the first spikes. Repetitive stimuli at 20 or 100 Hz also caused progressive use-dependent depression during the train. Notably, veratridine, an inhib...Continue Reading

Citations

Mar 21, 2019·Journal of Neurophysiology·Shailesh N KhatriJason R Pugh
May 21, 2019·Frontiers in Cellular Neuroscience·Mickael Zbili, Dominique Debanne
Sep 27, 2019·Frontiers in Cellular Neuroscience·Haruyuki Kamiya
May 30, 2019·Frontiers in Cellular Neuroscience·Haruyuki Kamiya

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