Short-term effects of centrally administered galanin on the hyperosmotically stimulated expression of vasopressin in the rat hypothalamus. An in situ hybridization and immunohistochemistry study

Neuroendocrinology
M LandryA Calas

Abstract

Galanin (GAL) is a 29-amino acid peptide that is present in the hypothalamic magnocellular neurons of the rat supraoptic nucleus (SON) and paraventricular nucleus (PVN). Since previous studies revealed a possible role of GAL in the hydro-osmotic regulation, we have investigated the effects of GAL on the vasopressin (AVP) mRNA content in the hypothalamic magnocellular neurons. We demonstrated by in situ hybridization (ISH) and immunohistochemistry that 100 pmol of GAL or GAL fragment (1-16) injected into the lateral ventricle of anesthetized dehydrated male rats induced a rapid (10 min time interval) decrease of AVP mRNA and AVP content in the magnocellular cell bodies of SON and PVN. These effects were quantified on an autoradiographic film and were also obvious at the cellular level by using ISH with a radiolabeled or digoxigenin-labeled oligonucleotide probe. Oxytocin mRNA content is not altered by the same injection either in dehydrated male or lactating female rats. Under the same conditions of lactation, AVP mRNA content is not modified and the i.c.v. injection of GAL has no effect. GAL antagonist (M15) injection suppressed the GAL-induced decrease of AVP mRNA in the dehydrated rats and increased AVP mRNA level in control ...Continue Reading

Citations

Jan 22, 2003·Physiology & Behavior·Ewa Bojanowska, Bozena Stempniak
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Feb 13, 2003·The European Journal of Neuroscience·Marc LandryAndré Calas
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Jun 20, 2003·The European Journal of Neuroscience·Marc LandryAndré Calas

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