PMID: 3766718Oct 1, 1986Paper

Short-term effects of PTH on cultured rat osteoblasts: changes in membrane potential

The American Journal of Physiology
A EdelmanS Balsan

Abstract

The introduction of parathyroid hormone [bPTH (1-34)], 10(-8) M, into the medium of cultured rat osteoblasts results in rapid (less than 1 min) depolarization of the osteoblast membranes. Conventional and pH-sensitive microelectrodes were used to assess the mechanism underlying this change. PTH depolarized cell membrane independently of steady-state membrane potential (Vm). Blocking K+ conductance (Ba2+) and Ca2+-dependent K+ conductance (quinine) depolarized Vm by +13.1 +/- 4.6 (n = 6) and +14.8 +/- 6.7 mV (n = 6), respectively, and both abolished the effect of PTH on Vm. The rate of depolarization was reduced in low-Ca2+ medium. PTH inhibited low Na+-induced cell hyperpolarization, but intracellular pH was not altered by hormone addition. PTH-induced depolarization occurred even when the Na+-K+ pump was blocked with ouabain. A second slower response was seen in cells having a Vm lower than -60 mV, with an increase in negativity 5-15 min after hormone application. The results indicate that PTH rapidly modifies Vm by changes of K+ conductance, which may be the first step in hormonal stimulus-response coupling, and induces delayed, long-term changes in cell status.

References

Apr 1, 1979·The Journal of Clinical Endocrinology and Metabolism·S R GoldringS M Krane
Feb 1, 1978·Journal of Dental Research·B G JeansonneW S Rehm
Apr 1, 1979·Journal of Dental Research·B G JeansonneW S Rehm
Dec 15, 1978·Pflügers Archiv : European journal of physiology·A EdelmanE Frömter
May 1, 1985·Pflügers Archiv : European journal of physiology·G MessnerF Lang
Jan 1, 1974·Annals of the New York Academy of Sciences·C D Cone
Oct 1, 1970·The American Journal of Physiology·K Hermsmeyer, N Sperelakis
Jan 1, 1980·Calcified Tissue International·D C WilliamsC C Johnston
Oct 1, 1981·Physiological Reviews·W R Loewenstein
Jan 1, 1981·Calcified Tissue International·W A PeckS Barr
Feb 23, 1984·Nature·O H Petersen, Y Maruyama
Jul 13, 1983·Biochimica Et Biophysica Acta·A EdelmanS Balsan
Nov 21, 1964·Nature·S NISHI, H SOEDA

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Citations

Oct 1, 1992·Calcified Tissue International·K SchirrmacherD Bingmann
Dec 1, 1990·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J H RaveslootP J Nijweide
Sep 1, 1991·The Journal of Membrane Biology·J FerrierS L Xia
Jul 1, 1990·Journal of Cellular Physiology·R MassassP Tetsch
Oct 1, 1991·Catheterization and Cardiovascular Diagnosis·E DomingoJ Soler-Soler
Feb 7, 2001·The Australian & New Zealand Journal of Obstetrics & Gynaecology·J F Morgan
Jan 27, 2006·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·M Harvie, A Howell
Jan 8, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R MoreauD Lajeunesse
Jun 1, 1996·International Journal of Sport Nutrition·C A DueckK S Matt
Oct 1, 1989·Pflügers Archiv : European journal of physiology·D Chesnoy-Marchais, J Fritsch
Dec 29, 1995·Annals of the New York Academy of Sciences·J E Nestler
Aug 1, 1990·Pflügers Archiv : European journal of physiology·R M DavidsonA L Auerbach
Oct 1, 1988·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J FritschS Balsan
Jul 1, 1993·Calcified Tissue International·K SchirrmacherD Bingmann
Jan 1, 1988·The Journal of Membrane Biology·D L YpeyP J Nijweide
Oct 28, 2020·Annual Review of Physiology·Geoffrey S PittChike Cao
Nov 1, 1996·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·T SugimotoH Yabu
Aug 1, 1989·Seminars in Arthritis and Rheumatism·D A Watrous, B S Andrews
Sep 1, 1989·Bone and Mineral·C GrygorczykJ Ferrier
Aug 1, 1997·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·M L Moss

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