PMID: 7581324Sep 1, 1995Paper

Short-term in vitro culture and molecular analysis of the microsporidian, Enterocytozoon bieneusi

The Journal of Eukaryotic Microbiology
G VisvesvaraC M Wilcox

Abstract

The microsporidium, Enterocytozoon bieneusi, causes a severe, debilitating, chronic diarrhea in patients with the acquired immunodeficiency syndrome. Specific diagnosis of intestinal microsporidiosis, especially due to Enterocytozoon, is difficult and there is no known therapy that can completely eradicate this parasite. Preliminary studies indicate that a short term (about 6 months) in vitro culture of this parasite yielding low numbers of spores, may be established by inoculating human lung fibroblasts and/or monkey kidney cell cultures with duodenal aspirates and or biopsy from infected patients. The cultures may subsequently be used for the isolation and molecular analysis of parasite DNA.

References

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Citations

Mar 20, 2012·Parasitology Research·Hend Aly El-TaweelMervat Mostafa Osman
Feb 3, 2009·In Vitro Cellular & Developmental Biology. Animal·S Richelle MonaghanNiels C Bols
Dec 4, 2001·International Journal for Parasitology·M T BonafonteJ R Mead
Jul 5, 2002·Clinical Microbiology Reviews·Govinda S Visvesvara
Jan 7, 2005·Journal of Clinical Microbiology·Abhineet S SheoranSaul Tzipori
Jul 1, 1996·Emerging Infectious Diseases·D P Fedorko, Y M Hijazi
Jan 16, 2007·Journal of Invertebrate Pathology·Mariano HigesAránzazu Meana
Apr 14, 2005·Enfermedades infecciosas y microbiología clínica·Jorge N VelásquezJosé M Peralta
Mar 7, 2001·Acta Tropica·L M Weiss
Jul 18, 2006·FEMS Microbiology Letters·Timothy R SouthernJ Russell Hayman
May 11, 2002·Critical Reviews in Microbiology·J Theron, T E Cloete
Mar 14, 2000·Veterinary Pathology·K Wasson, R L Peper
Jan 31, 2006·The Journal of Eukaryotic Microbiology·María HaroNuno Henriques-Gil
Dec 29, 2000·The Journal of Eukaryotic Microbiology·M ScanlonG J Leitch
Jul 3, 2003·The Journal of Eukaryotic Microbiology·Hercules MouraGovinda S Visvesvara
Jul 28, 2020·Journal of Fungi·Alexandra Valenčáková, Monika Sučik
Jul 1, 2021·Clinical Microbiology Reviews·Bing HanLouis M Weiss

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