PMID: 326796Jun 1, 1977Paper

Short term kinetics of luteinizing hormone secretion studied in dissociated pituitary cells attached to manipulable substrates

The Journal of Cell Biology
C R Hopkins

Abstract

With the use of poly-L-lysine, a method has been developed which induces acutely dissociated rat anterior pituitary cells to attach to glass and polyacrylamide surfaces. In these attached cells the recovery of the secretory response, which is impaired in acutely dissociated cells, has been followed, and it has been established that, in terms of their ability to secrete luteinizing hormone (LH) in response to the specific secretogogue luteinizing-hormone-releasing hormone (LHRH), the cells become maximally responsive after 48 h. The attached cells also allow the short-term kinetics of LH secretion to be followed with great facility; and, when cells allowed to recover for 48 h are used, it is shown that in response to LHRH the pattern of LH release is biphasic.

References

Jun 1, 1975·Proceedings of the Society for Experimental Biology and Medicine·J C Marshall, W D Odell
Jan 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J R GavinD N Buell
Nov 1, 1973·The Journal of Cell Biology·C R Hopkins, M G Farquhar
Dec 1, 1972·Proceedings of the Society for Experimental Biology and Medicine·W C HymerJ S Haskill
Jun 18, 1971·Biochemical and Biophysical Research Communications·H MatsuoA V Schally
Nov 1, 1968·Endocrinology·S E MonroeA R Midgley

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Citations

Nov 1, 1978·Molecular and Cellular Endocrinology·C R Hopkins, A M Walker
Oct 1, 1982·Molecular and Cellular Endocrinology·I M Kramer, C R Hopkins
Sep 1, 1989·Molecular and Cellular Endocrinology·N W FahmyG A Bourne
Dec 1, 1984·Clinics in Obstetrics and Gynaecology·S J Nillius
May 15, 1979·Biochemistry·M Liakopoulou-Kyriakides, R E Galardy
May 22, 1980·Nature·C DenefR Dewals
Jan 17, 1980·Biochimica Et Biophysica Acta·J C Zolman, L J Valenta
Apr 1, 1978·Molecular and Cellular Endocrinology·J R ReelR A Edgren

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