Short-Term Molecular Acclimation Processes of Legume Nodules to Increased External Oxygen Concentration

Frontiers in Plant Science
Ulrike AvenhausJoachim Schulze

Abstract

Nitrogenase is an oxygen labile enzyme. Microaerobic conditions within the infected zone of nodules are maintained primarily by an oxygen diffusion barrier (ODB) located in the nodule cortex. Flexibility of the ODB is important for the acclimation processes of nodules in response to changes in external oxygen concentration. The hypothesis of the present study was that there are additional molecular mechanisms involved. Nodule activity of Medicago truncatula plants were continuously monitored during a change from 21 to 25 or 30% oxygen around root nodules by measuring nodule H2 evolution. Within about 2 min of the increase in oxygen concentration, a steep decline in nitrogenase activity occurred. A quick recovery commenced about 8 min later. A qPCR-based analysis of the expression of genes for nitrogenase components showed a tendency toward upregulation during the recovery. The recovery resulted in a new constant activity after about 30 min, corresponding to approximately 90% of the pre-treatment level. An RNAseq-based comparative transcriptome profiling of nodules at that point in time revealed that genes for nodule-specific cysteine-rich (NCR) peptides, defensins, leghaemoglobin and chalcone and stilbene synthase were signific...Continue Reading

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Citations

Aug 16, 2016·Frontiers in Plant Science·Manuel González-GuerreroManuel Tejada-Jiménez
Jul 22, 2017·Plant, Cell & Environment·Isidro AbreuManuel González-Guerrero
Jan 20, 2018·The New Phytologist·Marta SenovillaManuel González-Guerrero
Sep 7, 2019·Plants·Andrés R SchwemberRicardo A Cabeza
Feb 23, 2020·Frontiers in Plant Science·Viviana EscuderoManuel González-Guerrero
Jun 4, 2019·Frontiers in Plant Science·Chiara PucciarielloPierdomenico Perata
Nov 7, 2019·International Journal of Molecular Sciences·Oldřich TrněnýDaniela Knotová
Aug 26, 2020·Journal of Experimental Botany·Rosario Castro-RodríguezManuel González-Guerrero

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Methods Mentioned

BETA
electrophoresis
PCRs
PCR

Software Mentioned

Statistica
DESeq

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