Short term optical defocus perturbs normal developmental shifts in retina/RPE protein abundance

BMC Developmental Biology
Nina RiddellSheila Gillard Crewther

Abstract

Myopia (short-sightedness) affects approximately 1.4 billion people worldwide, and prevalence is increasing. Animal models induced by defocusing lenses show striking similarity with human myopia in terms of morphology and the implicated genetic pathways. Less is known about proteome changes in animals. Thus, the present study aimed to improve understanding of protein pathway responses to lens defocus, with an emphasis on relating expression changes to no lens control development and identifying bidirectional and/or distinct pathways across myopia and hyperopia (long-sightedness) models. Quantitative label-free proteomics and gene set enrichment analysis (GSEA) were used to examine protein pathway expression in the retina/RPE of chicks following 6 h and 48 h of myopia induction with - 10 dioptre (D) lenses, hyperopia induction with +10D lenses, or normal no lens rearing. Seventy-one pathways linked to cell development and neuronal maturation were differentially enriched between 6 and 48 h in no lens chicks. The majority of these normal developmental changes were disrupted by lens-wear (47 of 71 pathways), however, only 11 pathways displayed distinct expression profiles across the lens conditions. Most notably, negative lens-wear...Continue Reading

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Citations

Feb 23, 2020·Annual Review of Analytical Chemistry·Brahim AchourJill Barber
Nov 5, 2019·Trends in Pharmacological Sciences·Tatiana V Tkatchenko, Andrei V Tkatchenko
Nov 1, 2020·Journal of Clinical Medicine·Emil Tomasz GrochowskiDiana Anna Dmuchowska
Jul 7, 2021·Experimental Eye Research·Jody A SummersAndrei V Tkatchenko
Aug 19, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cindy KaroutaRegan Ashby
Dec 28, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kate ThomsonRegan Ashby

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Methods Mentioned

BETA
GTPase
geranylgeranylation
GTPases
amino acid exchanger
gene knockout
x-ray microanalysis

Software Mentioned

Gene Set Enrichment Analysis ( GSEA )
imputeLCMD R
Cytoscape Network Analyser
InParanoid
LIMMA
Protein Interaction Network Analysis platform ( PINA ) Cytoscape
MaxQuant
GSEA
Andromeda
Cytoscape Enrichment Map app

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