PMID: 6983438Oct 1, 1982Paper

Short-term regulation of the nitrogenase activity in Rhodopseudomonas sphaeroides

European Journal of Biochemistry
H HaakerC Veeger

Abstract

The nitrogenase activity in whole cells of Rhodopseudomonas sphaeroides could be inhibited by lowering the electrical potential across the cytoplasmic membrane. The membrane potential was partly dissipated either by lowering the light intensity or by the addition of a lipophilic cation, tetraphenylphosphonium. Under these circumstances, it was shown that the intracellular ATP/ADP ratio was not affected and that the inhibition of the whole cell nitrogenase activity was not due to an inactivation of the nitrogenase enzyme. From these results it is concluded that electron transport to nitrogenase in Rps. sphaeroides is dependent on a high membrane potential. The nitrogenase enzyme in whole cells could be inactivated by lowering the membrane potential across the cytoplasmic membrane by incubating the cells in the dark or in the light in the presence of uncouplers. Nitrogenase could be reactivated in the light in the absence of uncouplers. Some possible mechanisms of action of NH+4 inhibition of whole cell nitrogenase activity could be excluded. Inhibition by NH4Cl of whole cell nitrogenase activity in Rps. sphaeroides could neither be explained by a rapid inactivation of the nitrogenase enzyme, nor by an effect on the intracellular...Continue Reading

References

Feb 1, 1979·Journal of Bacteriology·R P CarithersD I Arnon
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P W Ludden, R H Burris
Jan 1, 1979·Annual Review of Biochemistry·L E Mortenson, R N Thorneley
Jan 15, 1978·FEBS Letters·J MeyerP M Vignais
Apr 27, 1978·Archives of Microbiology·W G Zumft, F Castillo
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Jul 1, 1971·Plant Physiology·R WangJ Myers

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Citations

Jan 1, 1987·Critical Reviews in Microbiology·P C Hallenbeck
Jan 15, 1986·European Journal of Biochemistry·S K Apte, J Thomas
Jun 1, 1989·Journal of Bacteriology·H Fu, R H Burris
Jan 1, 1984·Journal of Bacteriology·J Klugkist, H Haaker
Dec 1, 1988·Applied and Environmental Microbiology·R M LankhorstR C van den Bos

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