Short-term tissue culture of prostatic carcinoma samples provides useful biological parameters related to patient prognosis

European Urology
M BolognaL Miano

Abstract

In order to identify new data to be able to better evaluate patient prognosis in prostatic carcinoma (PRCA), we started a study 6 years ago correlating in vitro parameters from human PRCA samples grown in tissue culture with histological diagnosis of the same tumors [Eur. Urol. 11: 330-333, 1985]. The original study has been extended with more cases and updated with follow-up of the patients. To date, we evaluated 51 specimens of PRCA (18 grade I, 19 grade II, 13 grade III and 1 grade IV) and 8 of benign prostatic hypertrophy. Tissue samples were cultured in medium DME plus 10% fetal calf serum, 10% horse serum and 50 ng/ml each of hydrocortisone and insulin. Epithelial cells grown from the explants showed an average life in culture and morphological and biochemical features in good correlation with tumor grade. Short cultural life span, regular growth and positive secretion activity are typical of low-grade tumors, meanwhile the opposite is true for high-grade tumors. Of these patients, 15 were evaluable for prognosis, because they died or because they were followed-up for at least 3 years. In this group we compared tissue culture data with survival and found a fairly good correlation between growth parameters and clinical out...Continue Reading

Citations

Jan 1, 1992·Journal of Cellular Biochemistry. Supplement·D G BostwickW Sakr
Jan 15, 2005·Journal of Cancer Research and Clinical Oncology·Claudio FestucciaMauro Bologna

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.