Short-term toxicity assessments of an antibiotic metabolite in Wistar rats and its metabonomics analysis by ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry

Toxicology and Applied Pharmacology
Hongxing HanZhenmei Lu

Abstract

4-Epi-oxytetracycline (4-EOTC), one of main oxytetracycline (OTC) metabolites, can be commonly detected in food and environment. The toxicity and effects of OTC on animals have been well characterized; however, its metabolites have never been studied systemically. This study aims to investigate 15-day oral dose toxicity and urine metabonomics changes of 4-EOTC after repeated administration in Wistar rats at daily doses of 0.5, 5.0 and 50.0mg/kg bw (bodyweight). Hematology and clinical chemistry parameters, including white blood cell count, red blood cell count, total protein, globulin and albumin/globulin, were obviously altered in rats of 5.0 and 50.0mg/kg bw. Histopathology changes of kidney and liver tissues were also observed in high-dose groups. Urinary metabolites from all groups were analyzed using ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). Seventeen metabolites contributing to the clusters were identified as potential biomarkers from multivariate analysis, including aminoadipic acid, 6-phosphogluconate, sebacic acid, pipecolic acid, etc. The significant changes of these biomarkers demonstrated metabonomic variations in treated rats, especially lys...Continue Reading

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Citations

Aug 9, 2019·Integrated Environmental Assessment and Management·Qi LiJiahua Guo
Feb 13, 2021·Environmental Pollution·Rayane Kunert LangbehnHugo Moreira Soares
Mar 8, 2019·Journal of Pharmaceutical and Biomedical Analysis·Mark A J BaylissAlasdair MacGowan
Nov 30, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mark A J BaylissAlasdair MacGowan
Oct 5, 2021·Frontiers in Veterinary Science·Bokyeong RyuC-Yoon Kim

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