PMID: 29762984Feb 1, 2017Paper

Short-term use of rapamycin combined with Tregs prolongs mouse cardiac graft survival but attenuates recipient’s anti-tumor immunity

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Ting YanShiqiao Luo

Abstract

To study the effect of short-term use of rapamycin( Rap) combined with regulatory T cells( Tregs)on the long-time survival of allogeneic mouse cardiac transplant,and its impact on the anti-tumor immunity of recipient. Mouse Tregs were purified from recipients’ spleen by magnetic activated cell sorting( MACS),and expanded by CD3 / CD28 monoclonal antibody immunomagnetic beads and 2000 U / m L recombinant mouse IL-2( rm IL-2) ex vivo. The purity was tested by fluorescence-activated cell sorting( FACS). Allogeneic mouse cardiac transplanted models were established( H-2~bto H-2~d),and the mice were divided into three groups: control group( transplant only),Rap group,and Rap combined with Tregs group. In the Rap group,the mice were treated with Rap [1 mg /( kg·d),ip] for 14 consecutive days,and the mice in the Rap plus Tregs group received the same treatment,and 1 × 107 Tregs were adoptively transferred through the tail vein on the day of transplantion. Meanwhile,the syngeneic transplanted group was set up( H-2~dto H-2~d). Allograft survival was monitored daily and the graft was harvested on the indicated day and histologically evaluated. In the experiment of recipient’s anti-tumor immunity,the similar three groups of allogeneic car...Continue Reading

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