PMID: 9419345Feb 21, 1998Paper

Shortage of mitogen-activated protein kinase is responsible for resistance to AP-1 transactivation and transformation in mouse JB6 cells

Proceedings of the National Academy of Sciences of the United States of America
C HuangZ Dong

Abstract

The JB6 mouse epidermal cell system, which includes tumor promotion-sensitive (P+) and tumor promotion-resistant (P-) cells, is a well-established and extensively used cell culture model for studying the mechanism of late-stage tumor promotion. Tumor promoters, such as 12-O-tetradecanoylphorbol 13-acetate (TPA) or epidermal growth factor (EGF), induce high levels of activator protein 1 (AP-1) activity and large, tumorigenic, anchorage-independent colonies in soft agar at a high frequency in JB6 P+ cells, but not in JB6 P- cells. We report here a molecular explanation for the defect in the AP-1 activation and promotion-resistant phenotype of P- cells. We demonstrate that the lack of AP-1 activation and cell transformation responses to TPA and EGF in P- cells appears attributable to the low level of mitogen-activated protein kinase (MAPK) (extracellular signal-regulated protein kinase, Erk) in these cells. TPA and EGF induce transactivation of AP-1 activity in P+ cells but not in P- cells. Nonphosphorylated forms and TPA- or EGF-induced phosphorylated forms of Erks (Erk1 and Erk2) in P- cells were much lower than those in P+ cells. Stable transfection of wild-type MAPK (Erk2) into P- cells restored its response to TPA and EGF for...Continue Reading

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Citations

Sep 30, 2004·International Journal of Cancer. Journal International Du Cancer·Farrukh AfaqHasan Mukhtar
Jul 31, 2013·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Lichao HouMin Ding
Sep 13, 2005·Phytochemistry·Masaaki NomuraKen-Ichi Miyamoto
Jan 14, 2003·Trends in Molecular Medicine·Matthew R YoungNancy H Colburn
Dec 6, 2012·Journal of Agricultural and Food Chemistry·Chiu-Mei LinKou-Gi Shyu
Nov 7, 2001·The Biochemical Journal·E V BerdyshevH H Schmid
Jun 4, 2008·The Journal of Biological Chemistry·Nam Joo KangZigang Dong
Jun 3, 2006·Cancer Research·Yasuaki TatsumiZigang Dong
May 20, 2006·American Journal of Respiratory Cell and Molecular Biology·Qiangsong TongDechun Li
Nov 12, 2002·Environmental Health Perspectives·Yi HuangChuanshu Huang
Oct 1, 2004·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Young-Joon Surh
Nov 1, 2011·Clinical Medicine : Journal of the Royal College of Physicians of London·Shruthi Konda
Jun 18, 2014·Toxicology and Applied Pharmacology·Sakshi MishraMukul Das
Apr 7, 2004·The Journal of Cell Biology·Jingxia LiChuanshu Huang
Nov 1, 2007·Expert Opinion on Investigational Drugs·Amit DeorukhkarBharat B Aggarwal
Oct 3, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Kausar M Ansari, Mukul Das
Nov 13, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Hideki MaeshimaToshihiro Yamada
Dec 18, 2009·The Journal of Investigative Dermatology·Thomas J WeberRyan D Quesenberry

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