Shot-noise limited Faraday rotation spectroscopy for detection of nitric oxide isotopes in breath, urine, and blood

Scientific Reports
Yin WangGerard Wysocki

Abstract

Measurement of NO and/or its metabolites in the various body compartments has transformed our understanding of biology. The inability of the current NO measurement methods to account for naturally occurring and experimental NO isotopes, however, has prevented the scientific community from fully understating NO metabolism in vivo. Here we present a mid-IR Faraday rotation spectrometer (FRS) for detection of NO isotopes. The instrument utilizes a novel dual modulation/demodulation (DM) FRS method which exhibits noise performance at only 2 times the fundamental quantum shot-noise level and provides the record sensitivity in its class. This is achieved with a system that is fully autonomous, robust, transportable, and does not require cryogenic cooling. The DM-FRS enables continuous monitoring of nitric oxide isotopes with the detection limits of 3.72 ppbv/Hz(1/2) to(14)NO and 0.53 ppbv/Hz(1/2) to(15)NO using only 45 cm active optical path. This DM-FRS measurement method can be used to improve the performance of conventional FRS sensors targeting other radical species. The feasibility of the instrument to perform measurements relevant to studies of NO metabolism in humans is demonstrated.

References

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Feb 8, 2013·Optics Express·Yin WangGerard Wysocki

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Citations

Jan 13, 2017·Optics Letters·Lukasz A SterczewskiGerard Wysocki
Aug 18, 2017·Chemical Society Reviews·Andreas SchwaighoferBernhard Lendl
May 3, 2020·The Journal of Chemical Physics·Jim C VisschersLykourgos Bougas
Nov 18, 2018·The Analyst·Katharina IsenseeWolfgang Petrich
Oct 16, 2018·Optics Letters·Jakob HaydenGerard Wysocki
Dec 17, 2017·Optics Express·Kuijun WuFaquan Li
Aug 10, 2017·The Journal of Chemical Physics·Michele GianellaGrant A D Ritchie
Feb 7, 2018·Optics Express·Karol KrzempekMichał Nikodem
Apr 16, 2021·Optics Letters·Marina Trad NeryBenno Willke
May 7, 2019··Gerard WysockiLink Patrick

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Methods Mentioned

BETA
circular dichroism

Software Mentioned

LabVIEW

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