Shotgun analysis of the marine mussel Mytilus edulis hemolymph proteome and mapping the innate immunity elements

Proteomics
Alexandre CamposSusana Cristobal

Abstract

The marine mussel innate immunity provides protection to pathogen invasion and inflammation. In this regard, the mussel hemolymph takes a main role in the animal innate response. Despite the importance of this body fluid in determining the physiological condition of the animal, little is known about the molecular mechanisms underlying the cellular and humoral responses. In this work, we have applied a MS (nano-LC-MS/MS) strategy integrating genomic and transcriptomic data with the aim to: (i) identify the main protein functional groups that characterize hemolymph and (ii) to map the elements of innate immunity in the marine mussel Mytilus edulis hemolymph proteome. After sample analysis and first protein identification based on MS/MS data comparison, proteins with unknown functions were annotated with blast using public database (nrNCBI) information. Overall 595 hemolymph proteins were identified with high confidence and annotated. These proteins encompass primary cellular metabolic processes: energy production and metabolism of biomolecules, as well as processes related to oxidative stress defence, xenobiotic detoxification, drug metabolism, and immune response. A group of proteins was identified with putative immune effector,...Continue Reading

References

Sep 1, 1997·Journal of Invertebrate Pathology·M J CarballalA Villalba
Oct 27, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·S TunkijjanukijJ A Olafsen
Oct 29, 2003·Marine Environmental Research·Allison C LuengenA Russell Flegal
Feb 6, 2004·Glycoconjugate Journal·Yehiel ZickDenise Ronen
Sep 2, 2006·Journal of Proteome Research·Boris ZybailovMichael P Washburn
Sep 12, 2006·Developmental and Comparative Immunology·M GonzalezE Bachère
Apr 7, 2007·Journal of Invertebrate Pathology·Saleem AladailehDavid A Raftos
Apr 21, 2009·Nature Methods·Jacek R WiśniewskiMatthias Mann
Jun 30, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kiyohiro TakahasiFuyuhiko Inagaki
Feb 18, 2010·Marine Drugs·Yong ZhaoRiccardo A A Muzzarelli
Apr 20, 2010·Journal of Proteome Research·Alfonsina D'AmatoPier Giorgio Righetti
May 29, 2013·Marine Pollution Bulletin·Aroon R MelwaniJay A Davis
Apr 15, 2014·Nature Biotechnology·Juan A VizcaínoHenning Hermjakob

❮ Previous
Next ❯

Citations

Jan 25, 2018·Marine Drugs·Dany Domínguez-PérezAgostinho Antunes
May 5, 2020·Environmental Microbiology·Delphine Destoumieux-GarzónLuigi Vezzulli
Mar 2, 2017·Proteomes·Alexandre M O Campos, André M de Almeida
Jun 7, 2021·Journal of Proteomics·Elisabetta GianazzaIngrid Miller

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.