Shotgun Lipidomics Combined with Laser Capture Microdissection: A Tool To Analyze Histological Zones in Cryosections of Tissues

Analytical Chemistry
Oskar KnittelfelderAndrej Shevchenko

Abstract

Shotgun analysis provides a quantitative snapshot of the lipidome composition of cells, tissues, or model organisms; however, it does not elucidate the spatial distribution of lipids. Here we demonstrate that shotgun analysis could quantify low-picomole amounts of lipids isolated by laser capture microdissection (LCM) of hundred micrometer-sized histological zones visualized at the cryosections of tissues. We identified metabolically distinct periportal (pp) and pericentral (pc) zones by immunostaining of 20 μm thick cryosections of a healthy mouse liver. LCM was used to ablate, catapult, and collect the tissue material from 10 to 20 individual zones covering a total area of 0.3-0.5 mm2 and containing ca. 500 cells. Top-down shotgun profiling relying upon computational stitching of 61 targeted selective ion monitoring ( t-SIM) spectra quantified more than 200 lipid species from 17 lipid classes including glycero- and glycerophospholipids, sphingolipids, cholesterol esters, and cholesterol. Shotgun LCM revealed the overall commonality of the full lipidome composition of pp and pc zones along with significant ( p < 0.001) difference in the relative abundance of 13 lipid species. Follow-up proteomics analyses of pellets recovered ...Continue Reading

References

Dec 13, 2002·Rapid Communications in Mass Spectrometry : RCM·Khalid A Al-SaadHerbert H Hill
Oct 28, 2003·Methods in Molecular Biology·R Gebhardt
Feb 24, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Stephan HailfingerMichael Schwarz
Oct 24, 2006·The FEBS Journal·Albert BraeuningMichael Schwarz
Jan 16, 2007·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Albert BraeuningMichael Schwarz
Apr 5, 2007·Nature Protocols·Virginia EspinaLance A Liotta
Sep 26, 2007·Expert Review of Molecular Diagnostics·Virginia EspinaLance A Liotta
Feb 19, 2008·Journal of Lipid Research·Vitali MatyashDominik Schwudke
Jan 29, 2009·Proceedings of the National Academy of Sciences of the United States of America·Christer S EjsingAndrej Shevchenko
Nov 17, 2009·The International Journal of Biochemistry & Cell Biology·Cyril TorreSabine Colnot
Jul 8, 2010·Nature Reviews. Molecular Cell Biology·Andrej Shevchenko, Kai Simons
Jul 21, 2010·Progress in Lipid Research·Beate FuchsJürgen Schiller
Jul 31, 2010·Journal of Lipid Research·Oswald QuehenbergerEdward A Dennis
Nov 13, 2010·World Journal of Gastroenterology : WJG·Elizabeth M Brunt, Dina G Tiniakos
Jan 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Julio L SampaioAndrej Shevchenko
Apr 23, 2011·Circulation. Cardiovascular Genetics·Christin StegemannManuel Mayr
May 26, 2011·Cold Spring Harbor Perspectives in Biology·Dominik SchwudkeAndrej Shevchenko
Jun 15, 2011·Biochimica Et Biophysica Acta·Melissa K Passarelli, Nicholas Winograd
Sep 29, 2011·Chemical Reviews·Karin A Zemski BerryRobert C Murphy
Jan 25, 2012·PloS One·Ronny HerzogAndrej Shevchenko
Jan 28, 2012·Journal of Mass Spectrometry : JMS·Kai SchuhmannAndrej Shevchenko
Apr 4, 2013·Journal of Lipid Research·Gerhard LiebischMichael J O Wakelam
Apr 30, 2013·Progress in Lipid Research·Shane R EllisTodd W Mitchell
Nov 23, 2013·Mass Spectrometry Reviews·Claudia BichAlain Brunelle
Feb 4, 2014·Neurobiology of Disease·Maï PanchalCharles Duyckaerts
Apr 2, 2014·Journal of Proteomics·Megan M GesselRichard M Caprioli
Jul 16, 2014·World Journal of Gastroenterology : WJG·Rolf Gebhardt, Madlen Matz-Soja
Sep 3, 2014·Journal of Cell Science·Masashi Maekawa, Gregory D Fairn
Jul 26, 2015·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Emine B Yalcin, Suzanne M de la Monte
Sep 24, 2015·Development·Oksana LavrynenkoAndrej Shevchenko
Oct 27, 2015·European Journal of Lipid Science and Technology : EJLST·Michal A SurmaJulio L Sampaio
Dec 26, 2015·Progress in Lipid Research·Miao WangXianlin Han

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Citations

Dec 11, 2019·Analytical and Bioanalytical Chemistry·Thomas ZülligHarald C Köfeler
Apr 2, 2020·Mass Spectrometry Reviews·Thomas Züllig, Harald C Köfeler
Mar 7, 2020·Proceedings of the National Academy of Sciences of the United States of America·Eylan YutucWilliam J Griffiths
Jul 28, 2020·Trends in Analytical Chemistry : TRAC·Jianing Wang, Xianlin Han
Mar 15, 2020·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Oskar KnittelfelderAndrej Shevchenko
Aug 14, 2021·Journal of Lipid Research·Olga VvedenskayaAndrej Shevchenko
Feb 2, 2022·Journal of the American Society for Mass Spectrometry·Chao DongKermit K Murray

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