Shotgun metabolomic approach based on mass spectrometry for hepatic mitochondria of mice under arsenic exposure

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
M A García-SevillanoJ L Gómez-Ariza

Abstract

Mass spectrometry (MS)-based toxicometabolomics requires analytical approaches for obtaining unbiased metabolic profiles. The present work explores the general application of direct infusion MS using a high mass resolution analyzer (a hybrid systems triple quadrupole-time-of-flight) and a complementary gas chromatography-MS analysis to mitochondria extracts from mouse hepatic cells, emphasizing on mitochondria isolation from hepatic cells with a commercial kit, sample treatment after cell lysis, comprehensive metabolomic analysis and pattern recognition from metabolic profiles. Finally, the metabolomic platform was successfully checked on a case-study based on the exposure experiment of mice Mus musculus to inorganic arsenic during 12 days. Endogenous metabolites alterations were recognized by partial least squares-discriminant analysis. Subsequently, metabolites were identified by combining MS/MS analysis and metabolomics databases. This work reports for the first time the effects of As-exposure on hepatic mitochondria metabolic pathways based on MS, and reveals disturbances in Krebs cycle, β-oxidation pathway, amino acids degradation and perturbations in creatine levels. This non-target analysis provides extensive metabolic i...Continue Reading

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Jan 23, 2014·Analytical and Bioanalytical Chemistry·M A García-SevillanoJ L Gómez-Ariza

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Citations

Feb 11, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·M A García-SevillanoJ L Gómez-Ariza
Jul 6, 2016·Journal of Ethnopharmacology·Taoguang HuoHong Jiang
Jun 25, 2020·Metabolomics : Official Journal of the Metabolomic Society·Gunter van der Walt, Roan Louw
Nov 19, 2019·BioMed Research International·Cesar Rivas-SantiagoYamilé López-Hernández
Oct 10, 2020·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Tong GuanXiujuan Zhao
Feb 11, 2021·Biological Trace Element Research·Chandra PrakashVijay Kumar
Mar 31, 2021·Environmental Science and Pollution Research International·Gema Rodríguez-MoroJosé-Luis Gómez-Ariza
Dec 21, 2016·Environmental Science & Technology·Jessica E LaineRebecca C Fry

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