Should platelet-rich plasma be activated in fat grafts? An animal study.

Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS
B HersantAnne Marie Rodriguez

Abstract

The adjunction of platelet-rich plasma with graft fat has been the subject of a few clinical trials which have demonstrated its value in adipocyte survival. The aim of this study was to assess the different efficacies between activated and non-activated PRP on adipose cells in vitro and for adipose tissue graft survival in vivo. The in vitro study assessed the effects of PRP on both the proliferation and adipocyte differentiation of adipose cells. For the in vivo study, 8 nude rats received 3 human fat injections as follows: 0.8 mL of fat + 0.2 mL of normal saline; 0.8 mL of fat + 0.2 mL of non-activated PRP; and 0.8 mL of fat + 0.2 mL of PRP activated with calcium chloride (CaCl2). The quantitative assessment of adipocyte survival was implemented after 3 months using histomorphometric analysis. Histological and immunohistochemical analysis were also performed to evaluate angiogenesis, inflammation and quality of adipocytes in the grafted tissue. We showed that activated PRP stimulated, in vitro, proliferation and differentiation of adipose cells. In vivo experiments indicated that CaCl2-activated PRP was more efficient than non-activated to prolong the survival of fat grafts in nude rats. The mean percentage areas occupied by ...Continue Reading

Citations

Nov 22, 2018·International Wound Journal·Oliver J SmithAsh Mosahebi
Nov 12, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Joong Il KimHyuk Soo Han
Dec 18, 2019·Aesthetic Plastic Surgery·Ruiquan LiuXian Zhao
Nov 13, 2019·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Amelia K Hausauer, Shannon Humphrey
Nov 27, 2019·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Amelia K Hausauer, Shannon Humphrey
Aug 26, 2019·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Maxime Abellan LopezFlorence Sabatier
Dec 1, 2020·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Tie-Lou ChenJin Liu

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