ShRNA-mediated Ku80 gene silencing inhibits cell proliferation and sensitizes to gamma-radiation and mitomycin C-induced apoptosis in esophageal squamous cell carcinoma lines

Journal of Radiation Research
Qing-Shan YangFeiyue Fan

Abstract

To investigate the effects of Ku80 depletion on cell growth and sensitization to gamma-radiation and MMC-induced apoptosis in esophageal squamous cell carcinoma lines. Six human carcinoma cell lines (LNcaP, K562, MDA-MB-231, MCF-7, EC9706, and K150) and normal HEK293 cell line were examined for basal levels of Ku80 protein by western blotting analysis. The suppression of Ku80 expression was performed using vector-based shRNA in EC9706 cells. Cell proliferation was determined with MTT assay and colony formation assay and tumorigenicity in a xenograft model in vitro and in vivo. Sensitivity of EC9706 cells treated with shRNA vector to gamma-radiation and MMC was determined with colony formation assay and MTT assay. The cell cycle distribution was determined by Flow cytometry. Apoptosis induced by gamma-radiation and MMC was analyzed using GENMED-TUNEL FACS kit. Ku80 showed higher basal levels in six carcinoma cell lines than in HEK293. The suppression of Ku80 expression decreased cellular proliferation, colony formation and inhibited tumorigenicity in a xenograft model. Furthermore, it sensitized apoptosis of the cancer cells induced by gamma-radiation and MMC. Ku80 plays an important role not only in tumorigenesis but also in ra...Continue Reading

References

Feb 15, 1988·International Journal of Cancer. Journal International Du Cancer·D M ParkinC S Muir
Sep 16, 1998·International Journal of Radiation Biology·E M Taylor, A R Lehmann
Apr 8, 2000·Critical Reviews in Biochemistry and Molecular Biology·R Tuteja, N Tuteja
Nov 23, 2000·Genes & Development·H L HsuD J Chen
Dec 6, 2000·British Journal of Cancer·C R WilsonC M West
May 22, 2002·International Journal of Radiation Biology·W K MyintG P Raaphorst
Jun 5, 2004·DNA Repair·Susan M BaileyEdwin H Goodwin
Jul 6, 2004·International Journal of Radiation Oncology, Biology, Physics·Timothy M Pawlik, Khandan Keyomarsi
Jul 29, 2004·DNA Repair·John Thacker, Małgorzata Z Zdzienicka
May 7, 2005·European Journal of Haematology·Tsai-Yun ChenChao-Jung Tsao
Feb 17, 2006·Biochimica Et Biophysica Acta·Charles GulloGerrard Teoh
May 13, 2006·International Journal of Radiation Biology·Eun Kyung ChoiHeon Joo Park

❮ Previous
Next ❯

Citations

Jul 26, 2011·The Journal of Urology·Hexing YuanJianquan Hou
Jun 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Prashant RaiBevin P Engelward
Mar 12, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Shuai WangZhenguo Sun
Apr 30, 2010·Molecular Neurobiology·Alison Colquhoun
Dec 18, 2012·International Journal of Molecular Medicine·Xin ZhaoHua Zhao
Mar 10, 2021·Journal of Cancer Research and Clinical Oncology·Hongfang ZhangShenglin Ma

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis