Shu1 is a cell-surface protein involved in iron acquisition from heme in Schizosaccharomyces pombe.

The Journal of Biological Chemistry
Thierry MourerSimon Labbé

Abstract

Iron is an essential metal cofactor that is required for many biological processes. Eukaryotic cells have consequently developed different strategies for its acquisition. Until now, Schizosaccharomyces pombe was known to use reductive iron uptake and siderophore-bound iron transport to scavenge iron from the environment. Here, we report the identification of a gene designated shu1(+) that encodes a protein that enables S. pombe to take up extracellular heme for cell growth. When iron levels are low, the transcription of shu1(+) is induced, although its expression is repressed when iron levels rise. The iron-dependent down-regulation of shu1(+) requires the GATA-type transcriptional repressor Fep1, which strongly associates with a proximal promoter region of shu1(+) in vivo in response to iron repletion. HA4-tagged Shu1 localizes to the plasma membrane in cells expressing a functional shu1(+)-HA4 allele. When heme biosynthesis is selectively blocked in mutated S. pombe cells, their ability to acquire exogenous hemin or the fluorescent heme analog zinc mesoporphyrin IX is dependent on the expression of Shu1. Further analysis by absorbance spectroscopy and hemin-agarose pulldown assays showed that Shu1 interacts with hemin, with a...Continue Reading

References

May 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·M T WorthingtonC L Berg
Apr 17, 2002·The Journal of Biological Chemistry·Benoit PelletierSimon Labbé
Sep 24, 2002·The Journal of Biological Chemistry·Daniel R BellemareSimon Labbe
Jan 17, 2003·Molecular Biology of the Cell·Dongrong ChenJürg Bähler
Mar 14, 2003·Trends in Biochemical Sciences·Resham D KulkarniRalph A Dean
Jul 8, 2003·Yeast·Piet W J De GrootFrans M Klis
Jul 30, 2004·International Journal of Food Microbiology·Ai Leng TeohJulian Cox
Feb 4, 2005·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Markus SchrettlHubertus Haas
Feb 22, 2005·Molecular Microbiology·Tianshun LianJames W Kronstad
May 4, 2005·The Journal of Biological Chemistry·Benoit PelletierSimon Labbé
May 24, 2006·The Journal of Biological Chemistry·Olga ProtchenkoCaroline C Philpott
Mar 11, 2008·Eukaryotic Cell·Olga ProtchenkoCaroline C Philpott
May 10, 2008·Molecular Microbiology·Ziva WeissmanDaniel Kornitzer
Jun 23, 2009·Current Opinion in Microbiology·Daniel Kornitzer
Sep 22, 2009·Chemical Reviews·Scott Severance, Iqbal Hamza
May 4, 2010·Applied and Environmental Microbiology·Alexandre Mercier, Simon Labbé
Aug 14, 2010·The Journal of Biological Chemistry·Elodie Desuzinges-MandonPierre Falson
Feb 7, 2012·Biochimica Et Biophysica Acta·Caroline C PhilpottAvery G Frey
May 12, 2012·Biochimica Et Biophysica Acta·Iqbal Hamza, Harry A Dailey
Jan 17, 2013·The Journal of Infectious Diseases·Brigitte CadieuxJames W Kronstad
Aug 7, 2013·Current Opinion in Microbiology·Simon LabbéJean-François Jacques
Aug 10, 2013·Current Biology : CB·Jerry Kaplan, Diane M Ward
Sep 7, 2013·PLoS Pathogens·James W KronstadWon Hee Jung
May 9, 2014·Metallomics : Integrated Biometal Science·Shane L HogleMartha Gledhill
May 17, 2014·PLoS Neglected Tropical Diseases·Elisa Flávia Luiz Cardoso BailãoCélia Maria de Almeida Soares
Jun 5, 2014·PloS One·Jean-François JacquesSimon Labbé
Jun 14, 2014·Frontiers in Pharmacology·Tamara Korolnek, Iqbal Hamza

❮ Previous
Next ❯

Citations

Mar 4, 2020·Cells·Samantha A SwensonOleh Khalimonchuk
Jul 29, 2016·G3 : Genes - Genomes - Genetics·Ariane BraultSimon Labbé
Mar 20, 2018·The Journal of Biological Chemistry·Vincent NormantSimon Labbé
Nov 22, 2019·Chemical Society Reviews·Toru ShimizuMarkéta Martínková
Mar 19, 2020·Current Genetics·Simon LabbéTobias Vahsen
Nov 4, 2020·Molecular Microbiology·Vincent NormantSimon Labbé
Jul 3, 2019·Current Opinion in Microbiology·Udita Roy, Daniel Kornitzer
Aug 10, 2018·Free Radical Biology & Medicine·Rebecca K DoneganAmit R Reddi
Aug 11, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Daniel Kornitzer, Udita Roy
Apr 4, 2021·Journal of Fungi·Janelle R RobinsonFelicia N Anike
Oct 7, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Ian G ChambersAmit R Reddi
Jul 15, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·María Teresa Martínez-Pastor, Sergi Puig

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Related Papers

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Simon LabbéAlexandre Mercier
Current Opinion in Microbiology
Simon LabbéJean-François Jacques
© 2022 Meta ULC. All rights reserved