Shugoshin biases chromosomes for biorientation through condensin recruitment to the pericentromere

ELife
Kitty F VerzijlbergenAdele L Marston

Abstract

To protect against aneuploidy, chromosomes must attach to microtubules from opposite poles ('biorientation') prior to their segregation during mitosis. Biorientation relies on the correction of erroneous attachments by the aurora B kinase, which destabilizes kinetochore-microtubule attachments that lack tension. Incorrect attachments are also avoided because sister kinetochores are intrinsically biased towards capture by microtubules from opposite poles. Here, we show that shugoshin acts as a pericentromeric adaptor that plays dual roles in biorientation in budding yeast. Shugoshin maintains the aurora B kinase at kinetochores that lack tension, thereby engaging the error correction machinery. Shugoshin also recruits the chromosome-organizing complex, condensin, to the pericentromere. Pericentromeric condensin biases sister kinetochores towards capture by microtubules from opposite poles. Our findings uncover the molecular basis of the bias to sister kinetochore capture and expose shugoshin as a pericentromeric hub controlling chromosome biorientation. DOI: http://dx.doi.org/10.7554/eLife.01374.001.

References

Jun 1, 1988·The Journal of Cell Biology·T C HuffakerD Botstein
Jun 1, 1995·The Journal of Cell Biology·M WineyJ R McIntosh
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·H J Yoon, J Carbon
Jan 31, 2004·Science·Adele L MarstonAngelika Amon
Aug 17, 2004·PLoS Biology·Stewart A WeberPaul C Megee
Aug 17, 2004·PLoS Biology·Earl F GlynnJennifer L Gerton
Oct 28, 2004·Cell·Silke Hauf, Yoshinori Watanabe
Mar 17, 2006·Nature·Tomoya S KitajimaYoshinori Watanabe
Feb 16, 2007·Molecular Biology of the Cell·Vincent VanoosthuyseKevin G Hardwick
Feb 27, 2007·Genes & Development·Shigehiro A KawashimaYoshinori Watanabe
Mar 21, 2007·The Journal of Cell Biology·Hong-Guo Yu, Douglas Koshland
Nov 6, 2007·Current Biology : CB·Vahan B Indjeian, Andrew W Murray
Dec 21, 2007·Molecular Biology of the Cell·Brendan M KiburzAdele L Marston
Feb 26, 2008·Journal of Structural Biology·Sophie Bachellier-BassiUlf Nehrbass
Aug 19, 2008·Genes & Development·Claudio D'AmbrosioFrank Uhlmann
Aug 22, 2008·Nature·Yuya YamagishiYoshinori Watanabe
Mar 6, 2009·Molecular Biology of the Cell·Susana A RibeiroPaola Vagnarelli
Mar 21, 2009·Genes & Development·Dean CliftAdele L Marston
Apr 17, 2009·Nature·Takeshi SakunoYoshinori Watanabe
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Jul 17, 2009·Molecular Biology of the Cell·Tessie M NgSue Biggins
Sep 1, 2009·Molecular Cell·Zheng XuWenqing Xu
Nov 17, 2009·Nature Methods·Kohei NishimuraMasato Kanemaki
Jan 19, 2010·Bioinformatics·Heng Li, Richard Durbin
Jan 30, 2010·Bioinformatics·Aaron R Quinlan, Ira M Hall
Aug 27, 2010·Nature·Tatsuya TsukaharaYoshinori Watanabe

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Citations

Nov 4, 2015·Frontiers in Oncology·Veronica Krenn, Andrea Musacchio
Oct 15, 2014·Developmental Cell·Judith H I HaarhuisBenjamin D Rowland
Jun 21, 2015·Current Opinion in Cell Biology·Yuri Frosi, Christian H Haering
Jun 4, 2015·ELife·Stephen M HinshawStephen C Harrison
Apr 15, 2016·Nature Reviews. Molecular Cell Biology·Frank Uhlmann
Jun 20, 2014·PLoS Genetics·Karolina PeplowskaZuzana Storchova
Jul 28, 2016·PLoS Genetics·Tyler W DoughtyJennifer A Benanti
Aug 20, 2016·Scientific Reports·Yuko Murakami-TonamiKenji Kadomatsu
Aug 22, 2017·Nature Cell Biology·Stephanie Andrea SchalbetterJonathan Baxter
Jul 12, 2018·Molecular Biology of the Cell·Emily L PettyLorraine Pillus
Dec 3, 2014·Molecular and Cellular Biology·Adele L Marston
Sep 27, 2018·G3 : Genes - Genomes - Genetics·Hemant Kumar PrajapatiSantanu K Ghosh
Aug 2, 2014·G3 : Genes - Genomes - Genetics·Cristina NogueiraTerry L Orr-Weaver
Jul 23, 2020·Molecular Biology of the Cell·Mary Kate BonnerAlexander E Kelly
Dec 11, 2019·International Journal of Molecular Sciences·Delaney Sherwin, Yanchang Wang
Jun 5, 2020·Nature·Flora PaldiAdele L Marston
Feb 8, 2020·Molecular Cell·Lise DaubanFrédéric Beckouët
Sep 23, 2014·Journal of Cell Science·Heather D Eshleman, David O Morgan
May 1, 2021·The Journal of Cell Biology·Xue Bessie SuAdèle L Marston

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Methods Mentioned

BETA
ChIP
FACS
PCR
immunoprecipitation
ChIP-Seq
pulldowns
co-immunoprecipitation

Software Mentioned

BWA
bedtools
microsoft excel
ImagePro
Integrated Genome Viewer

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