Si-CSP9 regulates the integument and moulting process of larvae in the red imported fire ant, Solenopsis invicta

Scientific Reports
Daifeng ChengXiaofang He

Abstract

Chemosensory proteins (CSPs) have been predicted to be involved in development; however, direct evidence for their involvement is lacking, and genetic basis is largely unknown. To determine the function of the chemosensory protein 9 (Si-CSP9) gene in Solenopsis invicta, we used RNA interference to silence Si-CSP9 in 3rd-instar larvae. The 3rd-instar larvae failed to shed their cuticle after being fed Si-CSP9-directed siRNA, and expression profiling of RNAi-treated and untreated control larvae showed that 375 genes were differentially expressed. Pathway enrichment analysis revealed that 4 pathways associated with larval development were significantly enriched. Blast analysis revealed that one fatty acid amide hydrolase (FAAH) gene was up-regulated and 4 fatty acid synthase (FAT) genes and one protein kinase DC2 gene (PKA) were down-regulated in the enriched pathways. Significantly higher expression of these genes was found in 4th-instar larvae, and Pearson correlation analysis of the expression patterns revealed significant relationships among Si-CSP9, PKA, FAAH, and FAT1-4. Moreover, we confirmed that expression levels of Si-CSP9, FAAH, and FAT1-4 were significantly reduced and that the development of 3rd-instar larvae was halt...Continue Reading

References

Jan 1, 1994·Critical Reviews in Biochemistry and Molecular Biology·P Pelosi
Sep 16, 2000·Insect Biochemistry and Molecular Biology·S MarcheseP Pelosi
Oct 30, 2001·Proteomics·G L Miklos, R Maleszka
Dec 4, 2001·Genes & Development·P W Ingham, A P McMahon
Oct 14, 2003·American Journal of Medical Genetics. Part a·M Michael Cohen
Feb 14, 2004·Bioinformatics·Tim Beissbarth, Terence P Speed
Jul 28, 2004·Journal of Chemical Ecology·Kevin W WannerErika Plettner
Oct 16, 2004·Archives of Insect Biochemistry and Physiology·Kalyani V P GunturRobert Renthal
Mar 2, 2005·Chemical Senses·Paolo PelosiLiping Ban
Jun 11, 2005·Science·Mamiko OzakiRyohei Yamaoka
Jun 15, 2005·Annual Review of Biochemistry·Michele K McKinney, Benjamin F Cravatt
Sep 15, 2005·The New Phytologist·Lee J Sweetlove, Alisdair R Fernie
Aug 4, 2006·Microscopy Research and Technique·Solange Marques-SilvaJosé Eduardo Serrão
Feb 14, 2007·Insect Biochemistry and Molecular Biology·Da-Ping GongZhong-Huai Xiang
Mar 5, 2008·Methods in Molecular Biology·Ghislain Bidaut
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Jun 16, 2009·Insect Molecular Biology·D GonzálezR Renthal
May 4, 2010·The Journal of Comparative Neurology·Aki NakanishiMichiko Nishikawa
Feb 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yannick WurmLaurent Keller
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
Jul 13, 2011·Nature Biotechnology·Matthew K Iyer, Arul M Chinnaiyan
Nov 26, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Yu-Ching Huang, John Wang
May 20, 2014·The Journal of Biological Chemistry·Bih-Hwa ShiehYuan Hong

❮ Previous
Next ❯

Citations

Apr 7, 2016·Annual Review of Neuroscience·D A Friedman, D M Gordon
May 20, 2016·Biology Letters·Jackson A HelmsEli S Bridge
Jul 23, 2020·Frontiers in Physiology·Yang ZengYoujun Zhang
Dec 20, 2019·Journal of Chemical Ecology·Yuan-Tao ZhouBao-Ping Pang
Feb 27, 2021·Bulletin of Entomological Research·Lu-Lu LiYa-Nan Zhang
May 10, 2017·Biological Reviews of the Cambridge Philosophical Society·Paolo PelosiFrancesca R Dani
Sep 11, 2021·Journal of Agricultural and Food Chemistry·Chunni ZhangWu Dai

❮ Previous
Next ❯

Datasets Mentioned

BETA
EE129471

Methods Mentioned

BETA
RNA-seq
electrophoresis
in vitro transcription

Software Mentioned

GOstat
RSEM
disSiRNA
Trinity
DESeq
Blast

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.