siah-1 Protein is necessary for high glucose-induced glyceraldehyde-3-phosphate dehydrogenase nuclear accumulation and cell death in Muller cells.

The Journal of Biological Chemistry
E Chepchumba K Yego, Susanne Mohr

Abstract

The translocation and accumulation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in the nucleus has closely been associated with cell death induction. However, the mechanism of this process has not been completely understood. The E3 ubiquitin ligase siah-1 (seven in absentia homolog 1) has recently been identified as a potential shuttle protein to transport GAPDH from the cytosol to the nucleus. Previously, we have demonstrated that elevated glucose levels induce GAPDH nuclear accumulation in retinal Müller cells. Therefore, this study investigated the role of siah-1 in high glucose-induced GAPDH nuclear translocation and subsequent cell death in retinal Müller cells. High glucose significantly increased siah-1 expression within 12 h. Under hyperglycemic conditions, siah-1 formed a complex with GAPDH and was predominantly localized in the nucleus of Müller cells. siah-1 knockdown using 50 nm siah-1 small interfering RNA significantly decreased high glucose-induced GAPDH nuclear accumulation at 24 h by 43.8 +/- 4.0%. Further, knockdown of siah-1 prevented high glucose-induced cell death of Müller cells potentially by inhibiting p53 phosphorylation consistent with previous observations, indicating that nuclear GAPDH induces...Continue Reading

References

Jul 1, 1993·Journal of Chemical Neuroanatomy·A ReichenbachL Hertz
Jun 1, 1996·Nature Medicine·D M Chuang, R Ishitani
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·M NemaniA Telerman
Aug 1, 1996·Vision Research·C Distler, Z Dreher
Jun 11, 1998·Neuroscience Letters·M Schütte, P Werner
May 18, 1999·Experimental Eye Research·R KannanN Kaplowitz
Dec 22, 1999·Oncogene·H Bruzzoni-GiovanelliF Calvo
May 10, 2001·Clinical & Experimental Ophthalmology·E LiethUNKNOWN Penn State Retina Research Group
Dec 14, 2001·Nature Structural Biology·Galina PolekhinaDavid D L Bowtell
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·L SusiniA Telerman
Dec 28, 2002·Journal of Neuroscience Research·Jennifer L Mazzola, Michael A Sirover
Mar 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Colin M HouseDavid D L Bowtell
Sep 25, 2003·The Journal of Biological Chemistry·Yoshito NaganoMasayasu Matsumoto
Dec 5, 2003·Oncogene·Antonia GermaniHeriberto Bruzzoni-Giovanelli
Feb 27, 2004·Investigative Ophthalmology & Visual Science·Tetsuya SugiyamaMasato Kobayshi
Feb 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Giusy FiucciAdam Telerman
Apr 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Esti LianiSimone Engelender
May 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Koki MoriyoshiShigetada Nakanishi
Aug 25, 2004·Investigative Ophthalmology & Visual Science·Pamela M MartinSylvia B Smith
Sep 17, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yunpeng DuT S Kern
Dec 15, 2004·Biochemical and Biophysical Research Communications·Xia XiJin-Zhong Zhang
Feb 3, 2005·Diabetologia·M M WardE L Fletcher
Apr 12, 2005·Annual Review of Pharmacology and Toxicology·De-Maw ChuangVladimir V Senatorov
Oct 27, 2005·Investigative Ophthalmology & Visual Science·Rachel A Feit-LeichmanDong Feng Chen
Mar 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Makoto R HaraSolomon H Snyder
Apr 18, 2006·Structure·Colin M HouseGalina Polekhina
Apr 25, 2006·Cellular and Molecular Neurobiology·Makoto R Hara, Solomon H Snyder
Jun 20, 2006·Experimental Eye Research·Nanami Adachi-UeharaMasaki Takiguchi
Dec 26, 2006·Biochemical and Biophysical Research Communications·Hiromasa FukubaMasayasu Matsumoto
Dec 26, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Frederick GroenmanMartin Post

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Citations

Jun 1, 2011·Journal of Ocular Biology, Diseases, and Informatics·Prathiba Jayaguru, Susanne Mohr
Aug 3, 2013·Science·Raymond E Moellering, Benjamin F Cravatt
Aug 13, 2011·American Journal of Physiology. Cell Physiology·Katherine E TruebloodGeorge R Dubyak
Sep 7, 2014·Neurotoxicology and Teratology·Haijing SunShao-Yu Chen
May 24, 2013·Cell Biochemistry and Biophysics·Jianfei QiZe'ev A Ronai
Jan 23, 2013·Molecular Neurobiology·Laura CampelloJosé Martín-Nieto
Sep 4, 2012·Journal of Molecular and Cellular Cardiology·Prasanth PuthanveetilBrian Rodrigues
Sep 28, 2010·Progress in Retinal and Eye Research·Chung-Jung Chiu, Allen Taylor
Jul 6, 2010·The International Journal of Biochemistry & Cell Biology·Mireia VenturaMaría Jesús Pujol
Feb 22, 2011·Seminars in Cell & Developmental Biology·Naila Rabbani, Paul J Thornalley
Jun 28, 2016·Trends in Biochemical Sciences·Aristeidis E BoukourisEvangelos D Michelakis
Apr 15, 2017·Biochemical Society Transactions·Moriah EusticeJohn A Hanover
Nov 19, 2011·Doklady. Biochemistry and Biophysics·E V SmirnovaV M Lipkin
May 7, 2020·Pharmaceutics·Vladimir F LazarevBoris A Margulis
Oct 31, 2017·Frontiers in Cellular Neuroscience·Taren Ong, David J Solecki
Oct 7, 2015·The Journal of Biological Chemistry·Sandra SuarezJohn S Penn
Jul 6, 2020·Antioxidants & Redox Signaling·Jennifer Selinski, Renate Scheibe
Dec 20, 2020·Antioxidants·Maria-Armineh TossounianIvan Gout
Feb 23, 2021·Biochemical Society Transactions·Warlen Pereira Piedade, Jakub K Famulski
Jul 25, 2019·Biochemical and Biophysical Research Communications·Brandon A CoughlinSusanne Mohr
Jul 9, 2021·Frontiers in Molecular Biosciences·Carolina Rodríguez-SaavedraSobeida Sánchez-Nieto
Aug 28, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Xiaoyu LiaoRui Liu

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