Sialic acid-binding lectins: submolecular specificity and interaction with sialoglycoproteins and tumour cells

Glycoconjugate Journal
E Fischer, R Brossmer

Abstract

We examined the specificity of limulin, Limax flavus agglutinin (LFA) and Sambucus nigra agglutinin I (SNA I) at the submolecular level of sialic acid, and characterized their interactions with a panel of structurally distinct sialoglycoproteins. In haemagglutination inhibition assays NeuAc-alpha-glycosides were stronger inhibitors for limulin and LFA than native N-acetylneuraminic acid (NeuAc). The N-acetyl of NeuAc was crucial for binding to both lectins. N-thioacetylated NeuAc lost affinity for LFA, but still bound to limulin. Thus, distinct intermolecular interactions are involved in binding of sialic acid to the lectins. The glyceryl side chain was required for interaction with LFA, but not with limulin. SNA I specifically bound NeuAc alpha 2 --> 6Gal beta 1 --> 4Glc, but not monomeric sialic acids. Limulin and LFA strongly interacted with O-chain glycoproteins, whereas SNA I preferred N-chain proteins that carry NeuAc alpha 2 --> 6 residues. The lectins were compared with those from Cepaea hortensis and Tachypleus tridentatus (TTA) and to wheat-germ agglutinin, and were then used to probe tumour cell lines for cell surface sialylation. With the exception of TTA, all lectins interacted with the tumour cells. Limulin distin...Continue Reading

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Citations

Jun 15, 1999·Biochimica Et Biophysica Acta·P BabálW A Gardner
May 31, 2012·The Journal of Biological Chemistry·Rafael S DepetrisJohn P Moore
Mar 25, 2004·FEMS Immunology and Medical Microbiology·Dieter GerlachKarl-Herman Schmidt
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Dec 26, 2001·The Journal of Biological Chemistry·Cornelia OetkeMichael Pawlita
Oct 1, 2021·ACS Chemical Biology·Landon J Edgar

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