Sialic acid-binding motif of Maackia amurensis lectins

Journal of Biochemistry
K YamamotoT Irimura

Abstract

Maackia amurensis hemagglutinin (MAH) and leukoagglutinin (MAL) are leguminous lectins which recognize carbohydrate chains containing sialic acid residues linked alpha2,3 to penultimate galactose residues. In the present investigation, cDNA clones encoding MAL were isolated from a cDNA library constructed from germinated Maackia amurensis seeds and sequenced. From the reading frame of the cloned cDNAs, MAL was predicted to be composed of 287 amino acid residues, and showed strong similarity to MAH (86.2% identity). In leguminous lectins, most amino acid residues involved in sugar-binding were previously shown to be conserved. However, in both MAL and MAH lectins, the conserved glycine and asparagine were shown to be substituted by lysine and aspartic acid, respectively. Substitutions were made at position 105 and/or 135 of MAH to examine the roles of amino acid residues postulated to be important in binding to sialic acids. Recombinant MAH bound to the sialic acid-containing CB-II glycopeptide of human glycophorin A. By contrast, mutant lectins with lysine-105 substituted with glycine and/or aspartic acid-135 with asparagine did not bind to sialic acid residues. This indicates that these characteristic substitutions are importa...Continue Reading

Citations

Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·M YimT Irimura
Mar 23, 2005·Experimental Eye Research·Carisa K MeagherThomas E Phillips
Jun 12, 2013·Biochemical and Biophysical Research Communications·Kazuya I P J HidariTakashi Suzuki
Oct 11, 2014·Organic & Biomolecular Chemistry·Gavin T NobleSimon J Webb
Jun 23, 2007·International Journal of Cancer. Journal International Du Cancer·Sylvie MathieuAssou El-Battari
Jun 20, 2007·Microscopy Research and Technique·Laura Gómez-SantosJuan Francisco Madrid
Apr 25, 2020·Biomolecules·Paula Carpintero-FernandezMaria D Mayan
Jul 27, 2017·Frontiers in Microbiology·Leonardo Freire-de-LimaLucia Mendonça-Previato
Jun 30, 2009·Journal of Medicinal Chemistry·Teruhiko MatsubaraToshinori Sato
Oct 3, 2008·Carbohydrate Research·Yoshimi HagaKenichi Hatanaka

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