Sialic Acid Ligand Binding of CD22 and Siglec-G Determines Distinct B Cell Functions but Is Dispensable for B Cell Tolerance Induction

The Journal of Immunology : Official Journal of the American Association of Immunologists
Lamia ÖzgörLars Nitschke

Abstract

Siglec-G and CD22 are inhibitory receptors on B cells and play an important role in the maintenance of tolerance. Although both molecules are expressed on all B cell populations at a similar level, Siglec-G was found to regulate exclusively B1a cells, whereas CD22 functions as an inhibitory receptor specifically on B2 cells. It is known that the mechanistic function of both Siglecs is regulated by sialic acid binding in a reciprocal manner, although it was not known until now how B cells would act when both Siglec-G and CD22 lack their ability to bind sialic acids. We answered this question by analyzing Siglec-G R120E x CD22 R130E mice. These mice show decreased numbers of mature recirculating B cells in the bone marrow similar to mice with mutations in CD22. Also, they show an increased B1a cell population in peritoneal cavity and a skewed BCR repertoire in peritoneal B1a cells, which is characteristic for mice with mutated Siglec-G. Ca2+ mobilization was strongly reduced in B2 cells and was altered in peritoneal B1a cells, whereas B cell survival was neither affected in B2 cells nor in B1a cells. Also, aging Siglec-G R120E x CD22 R130E mice do neither develop a general hyperactivated immune status nor autoimmunity. This demon...Continue Reading

References

Nov 1, 1996·Science·T L O'KeefeM S Neuberger
Feb 1, 1997·Current Biology : CB·L NitschkeM C Lamers
Sep 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·C MaryL Reininger
Jun 11, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Frédéric LajauniasShozo Izui
Oct 17, 2003·The New England Journal of Medicine·Melissa R ArbuckleJohn B Harley
Apr 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Brian E CollinsJames C Paulson
Dec 22, 2005·Nature Immunology·Brian E CollinsJames C Paulson
Jan 13, 2006·Nature Chemical Biology·Shoufa HanJames C Paulson
Mar 24, 2007·Nature Reviews. Immunology·Paul R CrockerAjit Varki
Apr 2, 2009·Nature Reviews. Genetics·Isaac T W HarleyJennifer A Kelly
Mar 5, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julia JellusovaLars Nitschke
Aug 24, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julia JellusovaLars Nitschke
Jan 10, 2012·Annual Review of Immunology·Shiv PillaiHamid Mattoo
Jan 1, 2011·Frontiers in Immunology·Julia Jellusova, Lars Nitschke
Jun 1, 2013·The Journal of Clinical Investigation·Matthew S MacauleyJames C Paulson
Jul 10, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fabian PfrengleJames C Paulson
Jul 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jennifer MüllerLars Nitschke
Jan 1, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chrystelle LamagnaClifford A Lowell
Mar 7, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Susanne BökersLars Nitschke
Apr 26, 2014·Nature Reviews. Rheumatology·Jennifer Müller, Lars Nitschke
May 3, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stefan HutzlerLars Nitschke
Sep 23, 2014·Nature Reviews. Immunology·Matthew S MacauleyJames C Paulson
May 20, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jennifer MüllerLars Nitschke
Dec 20, 2015·Immunological Reviews·Vinay S Mahajan, Shiv Pillai
Feb 8, 2018·Current Opinion in Immunology·Taras KreslavskyMeinrad Busslinger

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Citations

Jan 28, 2020·Annual Review of Immunology·Shiteng Duan, James C Paulson
Dec 19, 2018·Frontiers in Immunology·Sarah J MeyerLars Nitschke
Dec 1, 2020·The FEBS Journal·Heinz LäubliShoib Sarwar Siddiqui

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