Sialic acid specificity of myelin-associated glycoprotein binding.

The Journal of Biological Chemistry
B E CollinsR L Schnaar

Abstract

Myelin-associated glycoprotein (MAG), a nervous system cell adhesion molecule, is an I-type lectin that binds to sialylated glycoconjugates, including gangliosides bearing characteristic structural determinants (Yang, L. J.-S., Zeller, C. B., Shaper, N. L., Kiso, M., Hasegawa, A., Shapiro, R. E., and Schnaar, R. L. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 814-818). Two cell adhesion systems, COS-1 monkey kidney fibroblasts transiently transfected to express MAG and Chinese hamster ovary (CHO) cells stably transfected to express MAG, were used to probe the structural specificity of MAG-ganglioside binding. Both cell types bound to the same gangliosides: GQ1b alpha (IV3NeuAc,III6NeuAc,II3(NeuAc)2Gg4Cer) > GT1b = GD1a > GM3 > GM1, GD1b, and GQ1b (the latter do not support adhesion). Binding was enhanced by pretreatment of MAG-expressing cells with neuraminidase. MAG-expressing Chinese hamster ovary cells bound directly to gangliosides resolved on thin layer chromatograms, allowing detection of MAG binding species in a mixture. The simplest ganglioside ligand for MAG was GM3 bearing N-acetylneuraminic acid, whereas GM3 bearing N-glycolylneuraminic acid did not support adhesion. Chemical modifications of N-acetylneuraminic acid resi...Continue Reading

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S SpiegelM Wilchek
Jan 31, 1992·Biochimica Et Biophysica Acta·H JarrellC W Grant
Feb 1, 1992·Glycobiology·A Varki
Jan 1, 1990·Annals of the New York Academy of Sciences·B D Trapp
Jan 1, 1990·Annals of the New York Academy of Sciences·J L SalzerJ Bell
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D TyrrellD Asa
Nov 1, 1991·Glycobiology·R L Schnaar
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M ArquintR Dunn
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B Seed, A Aruffo
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C LaiJ G Sutcliffe
Oct 1, 1989·Journal of Neuroscience Research·G SpagnolN Latov
Jan 1, 1980·Advances in Experimental Medicine and Biology·L Svennerholm
May 1, 1984·Journal of Biochemistry·S Handa, K Nakamura
Jan 1, 1982·Advances in Carbohydrate Chemistry and Biochemistry·R Schauer
Jan 18, 1980·Biochimica Et Biophysica Acta·L Svennerholm, P Fredman
Feb 1, 1980·Journal of Neurochemistry·G H De Vries, C J Zmachinski
Sep 1, 1993·The European Journal of Neuroscience·T FahrigM Schachner
Jan 1, 1994·Methods in Enzymology·R L Schnaar, L K Needham
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·A Varki
Oct 20, 1994·Nature·M E Schwab, C E Bandtlow
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K E NorgardN M Varki
Feb 19, 1993·Brain Research. Developmental Brain Research·E BorroniV P Whittaker
Jun 16, 1995·The Journal of Biological Chemistry·L D Powell, A Varki
Jan 1, 1994·Progress in Brain Research·L Svennerholm
Jan 1, 1994·Methods in Enzymology·A DellH R Morris
Jan 1, 1994·Methods in Enzymology·R L Schnaar
Jan 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·L J YangR L Schnaar

❮ Previous
Next ❯

Citations

May 23, 2001·Archives of Biochemistry and Biophysics·T ArigaT Miyatake
Feb 4, 2010·Molecular Neurobiology·Marco PiccininiAlessandro Prinetti
Aug 8, 2001·Journal of the Neurological Sciences·K MizutaniH Shibasaki
Feb 27, 2001·Brain Research. Molecular Brain Research·P KursulaA M Heape
Aug 28, 2001·Biochimie·A Varki
May 25, 1999·Progress in Neurobiology·R Probstmeier, P Pesheva
Mar 23, 2000·Progress in Neurobiology·B GarbayC Cassagne
Sep 12, 2002·Biochimica Et Biophysica Acta·Takashi Angata, Els Brinkman-Van der Linden
Dec 5, 2002·Current Opinion in Structural Biology·Maria Laura Allende, Richard L Proia
Jun 28, 2012·Glycobiology·Elizabeth R SturgillRonald L Schnaar
Jun 14, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Richard L Proia
Apr 13, 2010·Annual Review of Biochemistry·Laura L Kiessling, Rebecca A Splain
Apr 5, 2003·Annual Review of Neuroscience·Andrea B HuberJean-François Cloutier
Jun 26, 2004·Annual Review of Neuroscience·Zhigang He, Vuk Koprivica
May 3, 2008·Chemical Reviews·Heather E Murrey, Linda C Hsieh-Wilson
Dec 17, 2008·Neurological Research·Lynda J-S Yang, Ronald L Schnaar
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·K A SheikhR L Schnaar
Jul 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lynda J S YangRonald L Schnaar
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·N Razi, A Varki
Feb 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tadashi YamashitaRichard L Proia
Oct 14, 2009·FEBS Letters·Ronald L Schnaar
Aug 30, 2011·Biochemical and Biophysical Research Communications·Nayanendu SahaDimitar B Nikolov
Jul 22, 2009·Molecular and Cellular Neurosciences·Zixuan CaoFrank S Walsh
Nov 22, 2000·Experimental Neurology·S ChiavegattoR L Schnaar
Feb 14, 2015·Biochimica Et Biophysica Acta·Massimo AureliAlessandro Prinetti
Jun 24, 2014·Neuroscience Research·Nayanendu SahaDimitar B Nikolov
Jun 5, 2016·Journal of Molecular Biology·Ronald L Schnaar
Apr 20, 2017·Chembiochem : a European Journal of Chemical Biology·Jonathan Okerblom, Ajit Varki
Apr 12, 2006·Chembiochem : a European Journal of Chemical Biology·Björn NeubacherJoachim Thiem
Dec 7, 2016·Nature Communications·Matti F PronkerBert J C Janssen

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.