Sialic acids regulate microvessel permeability, revealed by novel in vivo studies of endothelial glycocalyx structure and function

The Journal of Physiology
Kai B BetteridgeAndrew H J Salmon

Abstract

We have developed novel techniques for paired, direct, real-time in vivo quantification of endothelial glycocalyx structure and associated microvessel permeability. Commonly used imaging and analysis techniques yield measurements of endothelial glycocalyx depth that vary by over an order of magnitude within the same vessel. The anatomical distance between maximal glycocalyx label and maximal endothelial cell plasma membrane label provides the most sensitive and reliable measure of endothelial glycocalyx depth. Sialic acid residues of the endothelial glycocalyx regulate glycocalyx structure and microvessel permeability to both water and albumin. The endothelial glycocalyx forms a continuous coat over the luminal surface of all vessels, and regulates multiple vascular functions. The contribution of individual components of the endothelial glycocalyx to one critical vascular function, microvascular permeability, remains unclear. We developed novel, real-time, paired methodologies to study the contribution of sialic acids within the endothelial glycocalyx to the structural and functional permeability properties of the same microvessel in vivo. Single perfused rat mesenteric microvessels were perfused with fluorescent endothelial ce...Continue Reading

References

Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G DekanM G Farquhar
Jan 1, 1989·Membrane Biochemistry·F F VargasM De Jesus
Mar 22, 1982·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·P GörögG V Born
Mar 1, 1984·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A L Baldwin, C P Winlove
Jul 1, 1980·Microvascular Research·F E Curry, C C Michel
Aug 26, 1999·Glycobiology·H GeyerH J Schnittler
Apr 6, 2000·American Journal of Physiology. Heart and Circulatory Physiology·V H Huxley, D A Williams
Sep 29, 2000·American Journal of Physiology. Heart and Circulatory Physiology·X HuS Weinbaum
Jun 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sheldon WeinbaumStephen C Cowin
Jun 24, 2003·Journal of the American Society of Nephrology : JASN·Marie Jeansson, Börje Haraldsson
Aug 2, 2003·American Journal of Physiology. Heart and Circulatory Physiology·F E CurryR H Adamson
Oct 18, 2003·Circulation Research·Jeffry A FlorianJohn M Tarbell
Apr 10, 2004·The Journal of Physiology·R H AdamsonF E Curry
Jun 29, 2004·Microvascular Research·Bingmei M Fu, Shang Shen
Apr 5, 2005·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Fitz-Roy E Curry
Jul 26, 2005·Atherosclerosis·Luis A CunibertiJosé P Werba
Apr 6, 2006·Journal of Internal Medicine·J M Tarbell, M Y Pahakis
Feb 13, 2007·Biochemical and Biophysical Research Communications·Manolis Y PahakisJohn M Tarbell
Mar 22, 2007·Annual Review of Biomedical Engineering·Sheldon WeinbaumEdward R Damiano
Oct 19, 2007·Journal of the American Society of Nephrology : JASN·Anurag SinghPeter W Mathieson
Jul 9, 2008·Trends in Molecular Medicine·Ajit Varki
Oct 9, 2008·Science Signaling·John M Tarbell, Eno E Ebong
Dec 17, 2008·Journal of the American Society of Nephrology : JASN·Marie JeanssonBörje Haraldsson
Feb 3, 2009·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Charles C Michel, Fitz-Roy E Curry
Apr 15, 2009·Physiology·János Peti-PeterdiSarah L Vargas
Jun 9, 2009·Free Radical Biology & Medicine·Robert KumagaiGhassan S Kassab
Mar 5, 2010·Cardiovascular Research·J Rodney Levick, C Charles Michel
Jun 15, 2010·Cardiovascular Research·John M Tarbell
Jul 28, 2010·Diabetes & Vascular Disease Research : Official Journal of the International Society of Diabetes and Vascular Disease·Mohammad NassimizadehGustav V R Born
Oct 20, 2011·Annals of Biomedical Engineering·F E Curry, R H Adamson
Nov 22, 2011·The Journal of Pathology·Andrew H J Salmon, Simon C Satchell
Nov 24, 2011·Critical Care : the Official Journal of the Critical Care Forum·Chiara AdembriAngelo R De Gaudio

❮ Previous
Next ❯

Citations

Apr 27, 2019·American Journal of Physiology. Heart and Circulatory Physiology·Xi Zhuo JiangKai H Luo
Sep 19, 2019·The Journal of General Virology·Diego A EspinosaEva Harris
Jun 4, 2019·Biorheology·Sara DesideriRebecca R Foster
Sep 26, 2020·Journal of Intensive Care·Maira Nilson BenattiCarlos Henrique Miranda
May 4, 2018·Frontiers in Medicine·Stefano Barelli, Lorenzo Alberio
Apr 9, 2019·Journal of the Royal Society, Interface·Viola IntroiniStefano Guido
Nov 2, 2019·International Journal of Molecular Sciences·Mariana Castro DiasBritta Engelhardt
May 31, 2020·Minerva cardioangiologica·Anastasia V PoznyakAlexander N Orekhov
Dec 12, 2020·Experimental Biology and Medicine·Aric F LogsdonMichelle A Erickson
Dec 31, 2020·CNS Neuroscience & Therapeutics·Fangfang ZhaoYumin Luo
Feb 11, 2021·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Ana R Santa-MariaMária A Deli
Mar 16, 2021·Frontiers in Cardiovascular Medicine·Johanna Hol FosseReidunn Edelmann
Apr 2, 2021·Seminars in Thrombosis and Hemostasis·Antonio Junior LepeddaMarilena Formato
Feb 10, 2020·The American Journal of Pathology·Matthew J ButlerSimon C Satchell
Mar 13, 2021·American Journal of Physiology. Heart and Circulatory Physiology·Hongyan KangXiaoyan Deng
Apr 9, 2021·The American Journal of Chinese Medicine·Jun-Yuan ZhangLian-Wen Qi
May 19, 2021·Tissue Barriers·Fruzsina R WalterMária A Deli
Dec 17, 2021·Frontiers in Medicine·Barbara J BallermannBörje Haraldsson

❮ Previous
Next ❯

Methods Mentioned

BETA
imaging techniques
electron microscopy
fluorescence collection
fluorescence microscopy
transmission electron microscopy
confocal
electron
confocal microscopy
light microscopy

Software Mentioned

Prism ( GraphPad
Adobe Photoshop
Nikon NIS Elements
FIJI ( Image J )

Related Concepts