PMID: 7335153Oct 1, 1981Paper

Sialosyltransferase activity and specificity in the biosynthesis in vitro of sialosylgalactosylceramide (GM4) and sialosyllactosylceramide (GM3) by rat astrocytes, neuronal perikarya, and oligodendroglia

Neurochemical Research
A StoffynW T Norton

Abstract

Total homogenates of astrocytes, neuronal perikarya, and oligodendroglia isolated from rat brain exhibited sialosyltransferase activity in the biosynthesis in vitro of GM3 from lac-cer and CMP-NeuNAc. This activity was highest in neuronal perikarya. In contrast, only oligodendroglia were found to be capable of catalyzing the transfer of NeuNAc from CMP-NeuNAc to gal-cer to form GM4. This activity was about three fold higher in oligodendroglia from 10-day-old rat brain than in those from 60-day-old animals; an intermediate activity was observed in oligodendroglia from 20-day-old rats. Purified preparations of myelin from 14-day-old rat cerebrum, cerebellum, and spinal cord did not show activity in the synthesis of either GM3 or GM4.

References

Sep 1, 1975·Journal of Neurochemistry·R K Yu, S I Yen
Dec 1, 1972·Hoppe-Seyler's Zeitschrift für physiologische Chemie·D H van den Eijnden, W van Dijk
Oct 1, 1973·Journal of Neurochemistry·R W LedeenL F Eng
Jul 1, 1974·Journal of Neurochemistry·R K YuL F Eng
Jan 1, 1980·Advances in Experimental Medicine and Biology·R W LedeenJ E Haley

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Citations

Feb 1, 1966·Journal of the American Oil Chemists' Society·P J Stoffyn
Apr 15, 1986·Biochimica Et Biophysica Acta·S DasguptaE L Hogan
Jan 1, 1986·Archives of Virology·K C McCullough
May 1, 1994·Journal of Neurochemistry·B FreischützR K Yu
Jan 1, 1985·Progress in Lipid Research·P S Sastry

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