Sialyllactose and Galactooligosaccharides Promote Epithelial Barrier Functioning and Distinctly Modulate Microbiota Composition and Short Chain Fatty Acid Production In Vitro

Frontiers in Immunology
R J Joost van NeervenR J Joost van Neerven

Abstract

Human milk oligosaccharides (HMO) and prebiotic oligosaccharides are proposed to confer several health benefits to the infant. They shape the microbiota, have anti-inflammatory properties, and support epithelial barrier functioning. However, in order to select the best oligosaccharides for inclusion in infant formulas, there is a need to increase our understanding of the specific effects of HMO and prebiotics on the host immune system. Therefore, we investigated the effects of the HMO sialyllactose (SL), and galactooligosaccharides (GOS) on epithelial barrier functioning, microbiota composition, and SCFA production. The effect of GOS and SL on epithelial barrier functioning and microbiota composition was investigated using in vitro models. Epithelial barrier function was investigated by transcriptome analysis of fully polarized Caco-2 cells exposed for 6 h to SL or GOS. In addition, epithelial cell growth, alkaline phosphatase production, and re-epithelization was studied. Further, we investigated the effect of SL and GOS on microbiota composition and SCFA production using in vitro fecal batch cultures. Transcriptome analysis showed that SL and GOS both induced pathways that regulate cell cycle control. This gene-expression pro...Continue Reading

References

Aug 1, 1982·Journal of Clinical Microbiology·G O GuerrantC W Moss
Jul 1, 1997·Metabolism: Clinical and Experimental·T M WoleverJ L Chiasson
Jan 7, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·B GalP Hueso
Apr 20, 2002·Journal of Pediatric Gastroenterology and Nutrition·G MoroG Boehm
Jul 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·John D Storey, Robert Tibshirani
Oct 25, 2003·European Journal of Clinical Nutrition·B Wang, J Brand-Miller
Oct 6, 2004·Genome Biology·Robert C GentlemanJianhua Zhang
Dec 31, 2004·Journal of Pediatric Gastroenterology and Nutrition·Jan KnolJohn Wells
May 2, 2006·Statistical Applications in Genetics and Molecular Biology·Gordon K Smyth
Jan 18, 2008·World Journal of Gastroenterology : WJG·Andreas Sturm, Axel U Dignass
Mar 12, 2008·Clinical Reviews in Allergy & Immunology·Carol L WagnerDonna Johnson
Oct 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Harry SokolPhilippe Langella
Jan 28, 2009·Journal of Pediatric Gastroenterology and Nutrition·Silvia FanaroEnrico Bertino
May 28, 2009·BMC Evolutionary Biology·Salvador Almagro-Moreno, E Fidelma Boyd
Feb 13, 2010·The Journal of Pediatrics·Allan Walker
Jun 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·June L Round, Sarkis K Mazmanian
Jul 29, 2010·Physiological Reviews·Inna SekirovB Brett Finlay
Aug 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·Angela M ZivkovicDavid A Mills
Sep 9, 2010·Proceedings of the National Academy of Sciences of the United States of America·Peter van BaarlenMichiel Kleerebezem
Dec 15, 2010·Journal of Pediatric Gastroenterology and Nutrition·Christian BraeggerUNKNOWN ESPGHAN Committee on Nutrition
Dec 21, 2010·Nature Immunology·Kendle M Maslowski, Charles R Mackay
Jan 21, 2011·The New England Journal of Medicine·Josef Neu, W Allan Walker
Jun 3, 2011·World Journal of Gastroenterology : WJG·Masahiro Iizuka, Shiho Konno
Jul 23, 2011·Journal of Integrative Bioinformatics·Ke LinJack A M Leunissen
Dec 2, 2011·Methods in Molecular Biology·Maria ForlenzaGeert F Wiegertjes
Dec 14, 2011·Nature Reviews. Immunology·Harald RenzMathias Hornef
Dec 26, 2012·Journal of Microbiological Methods·S E LadiratF H J Schuren

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Citations

Sep 12, 2020·Critical Reviews in Food Science and Nutrition·Emmanuel Pérez-EscalanteLuis Guillermo González-Olivares
Apr 25, 2020·The American Journal of Clinical Nutrition·Lauren R BrinkLaxmi Yeruva
Apr 4, 2021·International Journal of Molecular Sciences·Suzanne AbbringBetty C A M van Esch
Jul 3, 2021·International Journal of Molecular Sciences·Elizabeth C RoseAmanda L Ziegler
Aug 17, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Tolulope Joshua Ashaolu
Apr 6, 2020·Food Research International·Iramaia Angelica Neri-Numa, Glaucia Maria Pastore
Jul 11, 2019·Journal of Agricultural and Food Chemistry·Gabriel López-GarcíaAmparo Alegría
Nov 4, 2020·Journal of Agricultural and Food Chemistry·Sander S van LeeuwenLubbert Dijkhuizen
Sep 4, 2021·Critical Reviews in Food Science and Nutrition·Valentina AmbrogiDouwe van Sinderen

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Methods Mentioned

BETA
chips
PCR
FCS
Assay
flow cytometry
chip

Software Mentioned

affyPLM
Bioconductor
BaseClear
FlowJo
CellProfiler
SPSS Statistics
limma
R
MADMAX Analysis
FCS Express

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