Siamese Crocodile White Blood Cell Extract Inhibits Cell Proliferation and Promotes Autophagy in Multiple Cancer Cell Lines

Journal of Microbiology and Biotechnology
Santi PhosriSompong Klaynongsruang

Abstract

Cancer represents one of the most significant threats to human health on a global scale. Hence, the development of effective cancer prevention strategies, as well as the discovery of novel therapeutic agents against cancer, is urgently required. In light of this challenge, this research aimed to evaluate the effects of several potent bioactive peptides and proteins contained in crocodile white blood cell extract (cWBC) against LU-1, LNCaP, PC-3, MCF-7, and CaCo-2 cancer cell lines. The results demonstrate that 25, 50, 100, and 200 μg/ml cWBC exhibits a strong cytotoxic effect against all investigated cell lines (IC₅₀ 70.34-101.0 μg/ml), while showing no signs of cytotoxicity towards noncancerous Vero and HaCaT cells. Specifically, cWBC treatment caused a significant reduction in the cancerous cells' colony forming ability. A remarkable suppression of cancerous cell migration was observed after treatment with cWBC, indicating potent antimetastatic properties. The mechanism involved in the cancer cell cytotoxicity of cWBC may be related to apoptosis induction, as evidenced by typical apoptotic morphology features. Moreover, certain cWBC concentrations induced significant overproduction of ROS and significantly inhibited the S-G₂/...Continue Reading

Citations

May 15, 2021·Clinical and Experimental Pharmacology & Physiology·Chui Yiu Bamboo ChookWing Tak Wong
Jan 16, 2022·European Journal of Pharmacology·Surachai MaijaroenNisachon Jangpromma

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