PMID: 11319650Apr 25, 2001Paper

Sibutramine metabolites increase glucose transport by cultured rat muscle cells

International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity
C J BaileyR B Jones

Abstract

The anti-obesity agent sibutramine, a serotonin and noradrenaline reuptake inhibitor (SNRI), has been shown to reduce insulin resistance and improve glycaemic control in obese-diabetic ob/ob mice and overweight type 2 diabetic patients. To investigate whether sibutramine or its metabolites act directly on muscle cells to improve glucose uptake and insulin action. Uptake of the non-metabolized glucose analogue 2-deoxyglucose was measured in cultured L6 rat muscle cells after incubation with sibutramine, its two pharmacologically active metabolites and related agents. Sibutramine itself (10(-8)-10(-6) M) did not significantly affect 2-deoxyglucose uptake during incubations up to 72 h. The primary amine metabolite M2 (10(-7) and 10(-6) M) increased basal and insulin-stimulated 2-deoxyglucose uptake (by 12% and 34%) after 24 h incubation. These effects of M2 were lost by 72 h incubation. However, the secondary amine metabolite M1 (10(-6) M) increased basal and insulin-stimulated 2-deoxyglucose uptake (by 50%) after 72 h incubation, although M1 was ineffective after 24 h. M2 stimulated 2-deoxyglucose uptake in the presence of LY-294,002 (an inhibitor of phosphatidylinositol 3-kinase) but the effect of M2 was inhibited by cytochalasi...Continue Reading

References

Sep 1, 1991·Clinical Pharmacology and Therapeutics·M WeintraubM L Scheinbaum
Apr 1, 1989·Diabetes Care·R G PestellJ D Best
Jan 1, 1988·Progress in Neuro-psychopharmacology & Biological Psychiatry·W R BuckettG P Luscombe
Oct 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·D Yaffe
Sep 23, 1997·Diabetologia·G D Holman, M Kasuga
Aug 15, 1998·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·C Day, C J Bailey
Apr 27, 1999·British Journal of Pharmacology·I P ConnoleyM J Stock
Mar 7, 2001·Diabetes, Obesity & Metabolism·M J BickerdikeC T Dourish

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Citations

Mar 20, 2003·Current Diabetes Reports·C J Bailey
Jul 23, 2009·The British Journal of Nutrition·Dan GaoClifford J Bailey
Dec 13, 2006·Diabetes & Vascular Disease Research : Official Journal of the International Society of Diabetes and Vascular Disease·Dawn K ColettaClifford J Bailey
May 1, 2007·Diabetes & Vascular Disease Research : Official Journal of the International Society of Diabetes and Vascular Disease·Clifford J Bailey
Jan 26, 2007·The American Journal of Nursing·Laura D Hoeman

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