Sickle erythrocytes adhere to fibronectin-thrombospondin-integrin complexes exposed by thrombin-induced endothelial cell contraction

Microvascular Research
A B Manodori

Abstract

Vascular damage appears to be associated with sickle erythrocyte (SS RBC) adherence to the endothelium. Thrombin, which has been found in abnormal levels in many sickle patients, causes endothelial cell (EC) retraction and increased SS RBC adherence, and SS RBC adhere in the gaps opened between the EC. Our objective was to elucidate the mechanism of adherence to activated EC monolayers and to determine whether the matrix proteins thrombospondin (TSP) and fibronectin (FN) are mediators of this adherence. Thrombin activation elicited the same 2.5-fold increase in adherence whether 10 or 35% of the matrix was exposed, and the majority of the RBC adhered at the edges of the EC regardless of the extent of matrix exposed. Using static adherence assays we investigated whether TSP, FN, or the integrins alpha(v)beta(3) and alpha(5)beta(1) mediated adherence. Blocking antibodies to any of these four had no effect on adherence to untreated monolayers. However, all the increased adherence elicited by thrombin was abrogated by each one, whereas control antibodies had no effect. Immunofluorescent microscopy demonstrated that both integrins were present on the luminal surface of confluent EC. Neither TSP nor FN was exposed in confluent cultur...Continue Reading

References

Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·V N SmirnovB V Shekhonin
Jun 1, 1985·American Journal of Hematology·J L WautierJ P Caen
May 1, 1980·The New England Journal of Medicine·R P HebbelM H Steinberg
Jan 1, 1996·Annual Review of Medicine·K K Wu, P Thiagarajan
Mar 1, 1997·The Journal of Laboratory and Clinical Medicine·R P Hebbel, G M Vercellotti
Nov 27, 1997·The New England Journal of Medicine·A SoloveyR P Hebbel
Mar 1, 1996·Current Opinion in Hematology·T M Wick, J R Eckman
May 16, 2000·Journal of Leukocyte Biology·G W SullivanJ Linden
Jul 18, 2000·The Surgical Clinics of North America·P K Kim, C S Deutschman

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Citations

Apr 15, 2005·Transfusion Medicine Reviews·Marilyn J Telen
May 27, 2008·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·R ZennadiM J Telen
Apr 12, 2016·Translational Research : the Journal of Laboratory and Clinical Medicine·Yunus AlapanUmut A Gurkan
Jul 8, 2009·Clinical Biochemistry·Nicola Conran, Fernando F Costa
May 23, 2007·Journal of Cellular Physiology·Assunta PandolfiMario Bonomini
May 22, 2016·Molecular and Cellular Biochemistry·Natalia Di PietroAssunta Pandolfi
Jun 14, 2002·FEBS Letters·David Lominadze, William L Dean

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