Side-Chain Amino Acid-Based Cationic Antibacterial Polymers: Investigating the Morphological Switching of a Polymer-Treated Bacterial Cell

ACS Omega
Ishita MukherjeePriyadarsi De

Abstract

Synthetic polymer-based antimicrobial materials destroy conventional antibiotic resistant microorganisms. Although these antibacterial polymers imitate the properties of antimicrobial peptides (AMPs), their effect on bacterial cell morphology has not been studied in detail. To investigate the morphology change of a bacterial cell in the presence of antimicrobial polymer, herein we have designed and synthesized side-chain amino acid-based cationic polymers, which showed efficient antibacterial activity against Gram-negative (Escherichia coli), as well as Gram-positive (Bacillus subtilis) bacteria. Morphological switching from a rod shape to a spherical shape of E. coli cells was observed by field emission-scanning electron microscopy analysis due to cell wall disruption, whereas the B. subtilis cell structure and size remained intact, but stacks of the cells formed after polymer treatment. The zone of inhibition experiment on an agar plate for E. coli cells exhibited drastic morphological changes at the vicinity of the polymer-treated portion and somewhat less of an effect at the periphery of the plate.

References

Mar 5, 2003·Pharmacological Reviews·Michael R Yeaman, Nannette Y Yount
May 25, 1958·The Journal of Biophysical and Biochemical Cytology·E KELLENBERGER, A RYTER
Jan 7, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·K Poole
Dec 2, 2004·Journal of the American Chemical Society·M Firat IlkerE Bryan Coughlin
Mar 24, 2005·Journal of the American Chemical Society·Kenichi Kuroda, William F DeGrado
Dec 13, 2006·Nature Biotechnology·Robert E W Hancock, Hans-Georg Sahl
Jan 9, 2007·Biomacromolecules·Philippe H SellenetJeffrey P Youngblood
Apr 12, 2007·Biomacromolecules·El-Refaie KenawyRoy Broughton
Nov 24, 2007·Journal of the American Chemical Society·Brendan P MowerySamuel H Gellman
Dec 28, 2007·Materials Science & Engineering. R, Reports : a Review Journal·Gregory J GabrielGregory N Tew
Oct 28, 2008·Biochimica Et Biophysica Acta·Katsumi Matsuzaki
Nov 21, 2008·Chemistry : a European Journal·Gregory J GabrielGregory N Tew
Jul 16, 2009·Journal of the American Chemical Society·Brendan P MowerySamuel H Gellman
Oct 1, 2009·Chemistry : a European Journal·Karen LienkampGregory N Tew
Jun 22, 2010·Applied Microbiology and Biotechnology·Edmund F Palermo, Kenichi Kuroda
Oct 19, 2010·Applied Microbiology and Biotechnology·Larisa Timofeeva, Natalia Kleshcheva
Dec 21, 2010·Biomacromolecules·Iva SovadinovaKenichi Kuroda
Dec 22, 2010·The Journal of Physical Chemistry. B·Edmund F PalermoKenichi Kuroda
Jul 12, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Eunkyung JiDavid G Whitten
Aug 19, 2011·Biomacromolecules·Yukari OdaKenichi Kuroda
Jul 24, 2012·Frontiers in Immunology·Jeffrey R BrenderAyyalusamy Ramamoorthy
Jul 31, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Jiaul HoqueJayanta Haldar
Jan 26, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Kamal BauriPriyadarsi De
Jun 21, 2013·Molecular Biology of the Cell·Christian ReimoldPeter L Graumann
Sep 7, 2013·Accounts of Chemical Research·Federica SgolastraGregory N Tew
Oct 9, 2013·Biomacromolecules·Katherine E S LocockMatthias Haeussler
Nov 28, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Sonu KumarPriyadarsi De
Apr 17, 2014·Advanced Materials·Huanxiang YuanShu Wang
May 24, 2014·Chemical Communications : Chem Comm·Ashish PuniaNan-Loh Yang
Mar 4, 2015·Current Pharmaceutical Design·Kezban UlubayramIpek Eroglu
Apr 17, 2015·ACS Chemical Biology·Michael J WilhelmHai-Lung Dai

❮ Previous
Next ❯

Citations

Apr 26, 2019·Biomaterials Science·Lakshmi Priya DattaTapan Kumar Das
Jun 6, 2018·Frontiers in Microbiology·Siva L S VelivelliDilip M Shah
Apr 28, 2018·Chemical Communications : Chem Comm·Mohini M KonaiJayanta Haldar
Jul 6, 2018·International Journal of Environmental Research and Public Health·Ana Maria Carmona-Ribeiro
Mar 20, 2021·ACS Infectious Diseases·Joshua B JonesShonna M McBride
Oct 26, 2018·Bioconjugate Chemistry·Ryosuke KimuraToshihisa Mizuno
Mar 7, 2020·ACS Applied Materials & Interfaces·Xiaoling HeDong-Sheng Wei

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence
NMR
transmission electron microscopy

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.