Side chain packing of the N- and C-terminal helices plays a critical role in the kinetics of cytochrome c folding

Biochemistry
W ColónH Roder

Abstract

The pairing of two alpha-helices at opposite ends of the chain is a highly conserved structural motif found throughout the cytochrome c family of proteins. It has previously been shown that association of the N- and C-terminal helices is a critical early event in the folding process of horse cytochrome c and is responsible for the formation of a partially folded intermediate (INC). In order to gain further insight into the structural and energetic basis of helix packing interactions and their role in folding, we prepared a series of horse cytochrome c variants in which Leu94, a critical residue at the helix contact site, was replaced by Ile, Val, or Ala. The Ile and Val substitutions resulted in minor changes in the stability of the native state, indicating that conservative mutations can be accommodated at the helix interface with only minor structural perturbations. In contrast, the L94A mutation resulted in a 3.5 kcal/mol decrease in unfolding free energy, suggesting that the smaller Ala side chain causes severe packing defects at the helix interface. The effect of these mutations on the kinetics of folding and unfolding as a function of denaturant concentration was studied by a systematic series of stopped-flow fluorescence...Continue Reading

References

Jan 1, 1992·Annual Review of Biophysics and Biomolecular Structure·J M Scholtz, R L Baldwin
Mar 5, 1992·Journal of Molecular Biology·T KiefhaberF X Schmid
Jul 20, 1990·Journal of Molecular Biology·G W BushnellG D Brayer
Nov 25, 1981·Journal of Molecular Biology·T Takano, R E Dickerson
Jan 5, 1981·Journal of Molecular Biology·C ChothiaD Richardson
Feb 5, 1993·Journal of Molecular Biology·M KataokaY Goto

❮ Previous
Next ❯

Citations

Jan 1, 1997·Protein Science : a Publication of the Protein Society·J S FetrowR E Burton
May 30, 1998·Protein Science : a Publication of the Protein Society·W A McGee, B T Nall
Dec 29, 1998·Protein Science : a Publication of the Protein Society·M C DemirelI Bahar
Aug 1, 2007·Journal of Biological Physics·A N MorozovS H Lin
Jul 16, 2003·Journal of Molecular Biology·Stefano GianniHeinrich Roder
Mar 19, 2002·Journal of Inorganic Biochemistry·Niels H AndersenJens Ulstrup
Feb 5, 2002·Biochimica Et Biophysica Acta·Konstantin S Vassilenko, Vladimir N Uversky
Mar 21, 2003·Biophysical Chemistry·Maurizio BrunoriCarlo Travaglini-Allocatelli
Aug 1, 1997·Current Opinion in Biotechnology·A R Clarke, J P Waltho
Feb 1, 1997·Current Opinion in Structural Biology·W A EatonJ Hofrichter
Feb 1, 1997·Current Opinion in Structural Biology·H Roder, W Colón
Apr 15, 2008·Quarterly Reviews of Biophysics·S Walter EnglanderMallela M G Krishna
May 11, 2006·Chemical Reviews·Heinrich RoderHong Cheng
Mar 21, 1998·Nature Structural Biology·S R Yeh, D L Rousseau
May 20, 1998·Nature Structural Biology·M C Shastry, H Roder
Dec 1, 1996·Nature Structural Biology·W Colón, H Roder
Mar 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Marie-Eve Aubin-TamKimberly Hamad-Schifferli
Feb 27, 1999·Protein Science : a Publication of the Protein Society·S E RankinT J Pinheiro
Jul 13, 2000·Protein Science : a Publication of the Protein Society·N Sanghera, T J Pinheiro
Jan 1, 1997·Annual Review of Biochemistry·M LevittJ Tsai
Apr 26, 2013·Cellular and Molecular Life Sciences : CMLS·Sobia ZaidiFaizan Ahmad
Jan 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mallela M G Krishna, S Walter Englander
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y Luo, R L Baldwin
Jan 3, 2001·Proceedings of the National Academy of Sciences of the United States of America·J RumbleyS W Englander
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·W A HouryH A Scheraga
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·T R SosnickS W Englander
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·C K ChanJ Hofrichter
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·M S KayR L Baldwin
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·L C Wu, P S Kim
Feb 13, 2001·The Journal of Peptide Research : Official Journal of the American Peptide Society·E BouchayerP Gans
Mar 25, 2006·Organic & Biomolecular Chemistry·Huw JonesAndrew D Miller
Sep 28, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Hulin TaiYasuhiko Yamamoto
Mar 13, 2014·Journal of Molecular Biology·Sagar V KathuriaOsman Bilsel
Sep 26, 2008·Biophysical Chemistry·Hamidur RahamanFaizan Ahmad
Jan 15, 2015·Protein Science : a Publication of the Protein Society·Masaru YamanakaShun Hirota

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.