Side effects of intra-gastric photodynamic therapy: an in vitro study

Journal of Photochemistry and Photobiology. B, Biology
Paola FaraoniFranco Fusi

Abstract

Since many years it has been acknowledged that some bacterial species, among which H. pylori, P. aeruginosa, P. acnes accumulate endogenous photosensitizers (PS) in the form of porphyrins. This makes antibacterial photodynamic therapy (PDT) easier to perform due to the possible avoidance of external PS. In this study, we focus on gastric infections associated with the presence of Helicobacter pylori (H. pylori), known to accumulate and release both protoporphyrin IX (PPIX) and coproporphyrins. PDT versus H. pylori can be carried out by modified endoscopes or by new ingestible luminous devices under development. In both cases of in vitro and in vivo applications, either for therapy (PDT) or diagnosis, scientific literature lacks studies on the possible side-effects of light treatments on the surrounding tissues. To this aim we evaluated in vitro side-effects due to a possible intrinsic photosensitivity of gastric mucosa or to a photosensitization by the PS released from the bacterium itself. Photo-toxicity studies were conducted on the AGS cell line (ATCC® CRL-1739™), commonly used as a model for the stomach mucosa tissue, considering PPIX as the photosensitizing agent. After first evaluations of PPIX dark toxicity, its uptake a...Continue Reading

Citations

Oct 19, 2019·Future Microbiology·Ilaria BaccaniAlberto Antonelli
Mar 11, 2020·Frontiers in Microbiology·Paola MoriciAntonella Sgarbossa
Dec 5, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·A GnerucciG Romano
Jun 3, 2021·Biomedicines·Ece Polat, Kyungsu Kang
Sep 28, 2021·Critical Reviews in Microbiology·Cláudia SousaLuís D R Melo

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