Side population (SP) cells isolated from fetal rat calvaria are enriched for bone, cartilage, adipose tissue and neural progenitors

Bone
Shulin ZhangJ E Aubin

Abstract

In some tissues, stem cells are enriched within the side population (SP) cells characterized by the efficient efflux of Hoechst 33342, but few data are yet available to address whether such is the case in bone tissue. When we Hoechst-stained and FACS-analyzed freshly isolated 20- or 21-day fetal rat calvaria (RC) cells, a small fraction of cells (0.15 +/- 0.05%) comprised of a distinct SP. When SP, non-SP and total/unfractionated (Total) RC cells were plated at a density of 30 cells per microtiter well, the percentage of wells containing bone-forming progenitors (CFU-O) was significantly higher in the SP compared to the non-SP or Total populations (13 +/- 4% vs. 1.8 +/- 0.4% and 0.7 +/- 0.4% respectively). The SP was also highly enriched for CFU-alkaline phosphatase (CFU-ALP) and CFU-fibroblast (CFU-F). While Dex increased the recruitment of CFU-O and CFU-F in the SP, it did not increase the frequency of CFU-ALP. Limiting dilution analysis showed a non-linear relationship between cell densities (1, 5, 10, 20 and 30 cells/microtiter well) and the frequency of readout CFU-O, CFU-ALP and CFU-F in all populations, suggesting a cell non-autonomous component to proliferation-differentiation of these progenitor types. When the develop...Continue Reading

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Citations

May 15, 2012·Circulation Research·Kazumasa UnnoRonglih Liao
Jun 15, 2007·FEBS Letters·Danièle AdamskiDidier Wion
Sep 24, 2008·Journal of Cellular Biochemistry·Flavia Q PirihSotirios Tetradis
Nov 6, 2008·Anatomia, histologia, embryologia·S KässmeyerM Bahramsoltani
Sep 6, 2007·Journal of Cellular Physiology·Hong-Yun Li, Xin-Fu Zhou
Apr 27, 2017·Journal of Medicinal Food·Paulina Delgado CuencaShirin Hooshmand
Feb 12, 2009·Reproductive Sciences·Irene Cervelló, Carlos Simón
Feb 12, 2013·Cell Transplantation·Tadashi NinomiyaHiroaki Nakamura
Feb 12, 2008·American Journal of Physiology. Heart and Circulatory Physiology·M PhoC A McCulloch
Nov 6, 2007·Current Opinion in Endocrinology, Diabetes, and Obesity

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