PMID: 7086408Jul 1, 1982Paper

Sidedness of phospholipid synthesis on brain membranes

Journal of Neurochemistry
M Butler, P Morell

Abstract

We have investigated the localization of the site of incorporation and the subsequent equilibration of newly synthesized phospholipids in brain membranes. Rats were injected intracranially with [3H]glycerol; the animals were killed at varying times afterwards, and microsomal fractions were isolated from the brains. In some cases, microsomes were subfractionated on sucrose gradients. Initially, most of the radioactive phosphatidylethanolamine appeared in a pool that reacted with the impermeable reagent trinitrobenzene sulfonic acid (TNBS). This probe presumably modified only the lipid on the outer face of microsomal vesicles (which may, in large part, consist of pinched-off endoplasmic reticulum). At 5 min after injection, the specific radioactivity of the TNBS-modified phosphatidylethanolamine (cytoplasmic face) was four times that of the unmodified (luminal or inner face) phosphatidylethanolamine. With time, the ratio of the specific activities in the modified and unmodified pools of phosphatidylethanolamine approached 1.0, with kinetics that suggested a half-time on the order of 30 min for in vivo conversion of the TNBS-accessible to the -inaccessible pool. This equilibration in specific activities could be the result of eith...Continue Reading

References

Feb 20, 1979·Biochimica Et Biophysica Acta·D G BishopL L van Deenen
Mar 1, 1977·The Journal of Cell Biology·O S Nilsson, G Dallner
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R SundlerP R Vagelos
May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J E Rothman, E P Kennedy
Jul 4, 1978·Biochimica Et Biophysica Acta·A M van den BesselaarL L van Deenen
Nov 1, 1977·Biochimica Et Biophysica Acta·J A Higgins, R M Dawson
Dec 1, 1974·The Journal of Cell Biology·Y TamaiM Satake
Dec 18, 1968·Biochimica Et Biophysica Acta·K Saito, K Mukoyama
Mar 30, 1971·Biochemistry·R D Kornberg, H M McConnell
May 1, 1972·Journal of Neurochemistry·M A Verity
Feb 1, 1974·Journal of Neurochemistry·J W GurdW J Moore
Apr 1, 1973·Journal of Neurochemistry·J A Benjamins, G M McKhann
Apr 13, 1971·Biochimica Et Biophysica Acta·R F ZwaalL L van Deenen
Jun 1, 1965·Journal of Neurochemistry·M L CuznerN A Gregson
Nov 8, 1965·Biochimica Et Biophysica Acta·A Fleck, D Begg
Jan 1, 1967·Biochimica Et Biophysica Acta·P CerlettiR Strom
Mar 1, 1980·Journal of Neurochemistry·A G Buchanan, J N Kanfer
Mar 25, 1956·The Journal of Biophysical and Biochemical Cytology·G E PALADE, P SIEKEVITZ

Citations

Jan 1, 1986·The Journal of Membrane Biology·L BinagliaG Porcellati
Jun 14, 1990·Biochimica Et Biophysica Acta·P BanerjeeG Dawson
Jan 1, 1984·Neurobiology of Aging·F Schroeder
Jul 4, 2001·Nature Reviews. Molecular Cell Biology·H SprongG van Meer
Jan 1, 1986·Journal of Neurochemistry·L CorazziG Arienti
Jul 1, 1985·Journal of Neurochemistry·L FreyszG Porcellati
Jan 1, 1985·Journal of Neurochemistry·G ArientiG Porcellati

Related Concepts

Brain
Cell Fractionation
Cell Membrane Permeability
Intracellular Membranes
Microsomes
Phosphatidylcholines
Phosphatidylethanolamines
Type C Phospholipases
Phospholipids
Drug or Chemical Tissue Distribution

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