Siderophore production of a periplasmic transport binding protein kinase gene defective mutant of Magnetospirillum magneticum AMB-1

Biochemical and Biophysical Research Communications
Ronie J CalugayTadashi Matsunaga

Abstract

A non-magnetic mutant, NMA61, of the magnetic bacterium Magnetospirillum magneticum AMB-1 was generated by transposon mutagenesis to identify genes involved in magnetosome synthesis. The genomic region of NMA61 interrupted by a Mini-Tn5 transposon was analyzed. The transposon was inserted in an open reading frame (ORF) coding for a periplasmic transport binding protein kinase gene homologue. Three adjacent ORFs and a promoter were identified upstream, indicating that the sequences comprised an operon. Phenotype characterizations showed that the growth inhibition imposed by the exogenous non-assimilable iron chelator nitrilotriacetate was relieved in wild type but not in NMA61, by the addition of the isolated wild type siderophore. Higher concentration of siderophores accumulated in the culture medium of NMA61 than in wild type. These data suggest that the interrupted periplasmic transport binding protein kinase gene homologue is required for siderophore transport into M. magneticum AMB-1.

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Citations

Jun 19, 2008·Journal of the Royal Society, Interface·Atsushi ArakakiTadashi Matsunaga
Jun 20, 2006·Journal of Bioscience and Bioengineering·Ronie J CalugayTadashi Matsunaga
May 15, 2012·Microbiological Research·Lei YanHongyu Li
Jun 10, 2008·FEMS Microbiology Reviews·Dirk Schüler
Apr 16, 2019·Frontiers in Microbiology·Agata Olszewska-WiddratDamien Faivre

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