Sif2p interacts with Sir4p amino-terminal domain and antagonizes telomeric silencing in yeast

Current Biology : CB
M CockellS M Gasser

Abstract

Several regions of the Saccharomyces cerevisiae genome are subject to position-dependent transcriptional repression mediated by a multi-component nucleosome-binding complex of silent information regulator proteins (Sir2p, Sir3p and Sir4p). These proteins are present in limiting amounts in the nucleus and are targeted to specific chromosomal regions by interaction with sequence-specific DNA-binding factors. Different sites of repression compete for Sir complexes, although it is not known how Sir distribution is regulated. In a screen for factors that interact with Sir4p amino terminus, we have cloned SIF2, which encodes a WD40-repeat-containing factor that disrupts telomeric silencing when overexpressed. In contrast to deletion of SIR4, SIF2 deletion improved telomeric repression, suggesting that under normal conditions Sif2p antagonizes Sir4p function at telomeres. Sif2p overexpression altered the subnuclear localization of Sir4p, but not its protein expression level, suggesting that Sif2p may recruit Sir4p to nontelomeric sites or repression. The sif2 mutant strains were hypersensitive to a range of stress conditions, but did not have decreased viability and did not alter repression in the rDNA. In conclusion, Sif2p resembles ...Continue Reading

References

Jan 15, 1997·Genes & Development·J S Smith, J D Boeke
Jun 1, 1997·Current Opinion in Cell Biology·M Grunstein

❮ Previous
Next ❯

Citations

Mar 31, 2010·Molecular Genetics and Genomics : MGG·Michihiko FujiiDai Ayusawa
Nov 28, 2001·Gene·S M Gasser, M M Cockell
Oct 18, 2000·The EMBO Journal·S J BoultonN Dyson
Dec 19, 2007·Molecular and Cellular Biology·Russell P DarstLorraine Pillus
Nov 2, 2001·Molecular and Cellular Biology·P Moretti, D Shore
Dec 8, 2004·Genetics·Michael AyeSuzanne Sandmeyer
Oct 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Shivkumar VenkatasubrahmanyamHiten D Madhani
Jan 23, 2016·Malaria Journal·Warren Antonio Vieira, Thérèsa L Coetzer
Jan 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Canan KuscuLeemor Joshua-Tor
Dec 30, 1999·The Journal of Cell Biology·K WeipoltshammerP Hozák
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·M CockellS M Gasser
Sep 22, 1999·Molecular and Cellular Biology·A Kahana, D E Gottschling
Mar 4, 2008·Trends in Plant Science·Zhongchi Liu, Vidyadhar Karmarkar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.