SIgA binding to mucosal surfaces is mediated by mucin-mucin interactions

PloS One
H L GibbinsG H Carpenter

Abstract

The oral mucosal pellicle is a layer of absorbed salivary proteins, including secretory IgA (SIgA), bound onto the surface of oral epithelial cells and is a useful model for all mucosal surfaces. The mechanism by which SIgA concentrates on mucosal surfaces is examined here using a tissue culture model with real saliva. Salivary mucins may initiate the formation of the mucosal pellicle through interactions with membrane-bound mucins on cells. Further protein interactions with mucins may then trigger binding of other pellicle proteins. HT29 colon cell lines, which when treated with methotrexate (HT29-MTX) produce a gel-forming mucin, were used to determine the importance of these mucin-mucin interactions. Binding of SIgA to cells was then compared using whole mouth saliva, parotid (mucin-free) saliva and a source of purified SIgA. Greatest SIgA binding occurred when WMS was incubated with HT29-MTX expressing mucus. Since salivary MUC5B was only able to bind to cells which produced mucus and purified SIgA showed little binding to the same cells we conclude that most SIgA binding to mucosal cells occurs because SIgA forms complexes with salivary mucins which then bind to cells expressing membrane-bound mucins. This work highlights ...Continue Reading

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Citations

Apr 19, 2017·Archives of Oral Biology·Christian HannigGuy Carpenter
Feb 16, 2017·Archives of Oral Biology·Jeong-Hyun KangHong-Seop Kho
Nov 7, 2015·PloS One·Eduardo B MoffaWalter L Siqueira
Jun 19, 2019·Bioinspiration & Biomimetics·Clementine PradalJason R Stokes
Dec 25, 2015·Journal of Oral Microbiology·Erica Shapiro Frenkel, Katharina Ribbeck
May 21, 2020·Macromolecular Bioscience·Carolin Alexandra RickertOliver Lieleg
May 26, 2017·Applied Microbiology and Biotechnology·Joanna Maria Radziwill-BienkowskaMagdalena Kowalczyk
May 5, 2018·Science·G P DonaldsonS K Mazmanian
May 16, 2020·Frontiers in Immunology·Andreas FreyMichael Wegmann
Aug 23, 2020·Journal of Clinical Medicine·Agostino Di CiaulaPiero Portincasa
Jul 31, 2018·AIDS Research and Human Retroviruses·Ester LopezAmy W Chung
Apr 15, 2020·Journal of Dental Research·G H Carpenter
Oct 18, 2019·Journal of Hepatology·Agustín AlbillosMaría Rescigno
Jun 3, 2021·International Journal of Molecular Sciences·Jasmin FlemmingMatthias Hannig

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
electrophoresis
confocal microscopy

Software Mentioned

Leica Confocal imaging

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