Sigma factor WhiGch positively regulates natamycin production in Streptomyces chattanoogensis L10

Applied Microbiology and Biotechnology
Shui-Ping LiuYong-Quan Li

Abstract

The roles of many sigma factors are unclear in regulatory mechanism of secondary metabolism in Streptomyces. Here, we report the regulation network of a group 3 sigma factor, WhiGch, from a natamycin industrial strain Streptomyces chattanoogensis L10. WhiGch regulates the growth and morphological differentiation of S. chattanoogensis L10. The whiG ch deletion mutant decreased natamycin production by about 30 % and delayed natamycin production more than 24 h by delaying the growth. Overexpression of the whiG ch gene increased natamycin production in large scale production medium by about 26 %. WhiGch upregulated the transcription of natamycin biosynthetic gene cluster and inhibited the expression of migrastatin and jadomycin analog biosynthetic polyketide synthase genes. WhiGch positively regulated natamycin biosynthetic gene cluster by directly binding to the promoters of scnC and scnD, which were involved in natamycin biosynthesis, and these binding sites adjacent to translation start codon were determined. Thus, this paper further elucidates the high natamycin yield mechanisms of industrial strains and demonstrates that a valuable improvement in the yield of the target metabolites can be achieved through manipulating the tran...Continue Reading

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Citations

Oct 30, 2015·Applied Microbiology and Biotechnology·Jesús F AparicioJavier Santos-Aberturas
Nov 11, 2016·Applied Microbiology and Biotechnology·Zhenhong ZhuYongquan Li
Mar 30, 2017·Applied Microbiology and Biotechnology·Kejing SongJianping Wen
Nov 23, 2016·Molecular Microbiology·Tan-Jun WangYong-Quan Li
Apr 14, 2016·Natural Product Reports·Mingzi M ZhangHuimin Zhao
Dec 19, 2016·F1000Research·Keith F Chater
Nov 22, 2019·Journal of Zhejiang University. Science. B·Zi-Yue LiYong-Quan Li
Dec 24, 2019·Current Protein & Peptide Science·Xin XieHui Jiang
Feb 4, 2019·Journal of Industrial Microbiology & Biotechnology·Yang ZhengXu-Ming Mao
Jan 10, 2018·Frontiers in Microbiology·Di SunWeijie Liu
Jan 5, 2021·Applied Microbiology and Biotechnology·Simona Barbuto FerraiuoloOdile Francesca Restaino
Jan 17, 2020·Biotechnology Advances·Amit SrivastavaRoman Sobotka

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